Cardiovascular Antibodies
Anti-TFPI Antibody (CAB1624)
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- SKU:
- CAB1624
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cardiovascular
Description
| Antibody Name: | Anti-TFPI Antibody |
| Antibody SKU: | CAB1624 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-224 of human TFPI (NP_001027452.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, MCF7, Mouse lung, Mouse heart, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-224 of human TFPI (NP_001027452.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DSEE DEEH TIIT DTEL PPLK LMHS FCAF KADD GPCK AIMK RFFF NIFT RQCE EFIY GGCE GNQN RFES LEEC KKMC TRDN ANRI IKTT LQQE KPDF CFLE EDPG ICRG YITR YFYN NQTK QCER FKYG GCLG NMNN FETL EECK NICE DGPN GFQV DNYG TQLN AVNN SLTP QSTK VPSL FVTK EGTN DGWK NAAH |
| Gene ID: | 7035 |
| Uniprot: | P10646 |
| Cellular Location: | GPI-anchor, Lipid-anchor, Microsome membrane, Secreted |
| Calculated MW: | 28kDa/35kDa |
| Observed MW: | 31kDa |
| Synonyms: | TFPI, EPI, LACI, TFI, TFPI1 |
| Background: | This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. |
| UniProt Protein Function: | Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma. |
| NCBI Summary: | This gene encodes a Kunitz-type serine protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. Inhibition of the encoded protein restores hemostasis in animal models of hemophilia. This gene encodes multiple protein isoforms that differ in their inhibitory activity, specificity and cellular localization. [provided by RefSeq, Jul 2016] |
| UniProt Code: | P10646 |
| NCBI GenInfo Identifier: | 125932 |
| NCBI Gene ID: | 7035 |
| NCBI Accession: | P10646.1 |
| UniProt Secondary Accession: | P10646,O95103, Q53TS4, |
| UniProt Related Accession: | P10646 |
| Molecular Weight: | 28,653 Da |
| NCBI Full Name: | Tissue factor pathway inhibitor |
| NCBI Synonym Full Names: | tissue factor pathway inhibitor |
| NCBI Official Symbol: | TFPI |
| NCBI Official Synonym Symbols: | EPI; TFI; LACI; TFPI1 |
| NCBI Protein Information: | tissue factor pathway inhibitor |
| UniProt Protein Name: | Tissue factor pathway inhibitor |
| UniProt Synonym Protein Names: | Extrinsic pathway inhibitor; EPI; Lipoprotein-associated coagulation inhibitor; LACI |
| Protein Family: | Tissue factor pathway inhibitor |
| UniProt Gene Name: | TFPI |
 | Western blot analysis of extracts of various cell lines, using TFPI antibody (CAB1624) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of MCF-7 cells using TFPI antibody (CAB1624). Blue: DAPI for nuclear staining. |
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