Immunology Antibodies 2
Anti-TFEB Antibody (CAB7311)
- SKU:
- CAB7311
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-TFEB Antibody |
| Antibody SKU: | CAB7311 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human TFEB. |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji, NIH/3T3, mouse spleen, mouse kidney, rat brain, rat spleen |
| Immunogen: | Recombinant protein of human TFEB. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 7942 |
| Uniprot: | P19484 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 43kDa/52kDa |
| Observed MW: | 70kDa |
| Synonyms: | TFEB, ALPHATFEB, BHLHE35, TCFEB |
| Background: |
| UniProt Protein Function: | TFEB: Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T- cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. Homodimer and heterodimer; with TFE3 or MITF. Belongs to the MiT/TFE family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p21 Cellular Component: cytoplasm; nucleus Molecular Function:transcription factor activity Biological Process: embryonic placenta development; humoral immune response; lysosome organization and biogenesis; positive regulation of autophagy; positive regulation of transcription from RNA polymerase II promoter |
| UniProt Code: | P19484 |
| NCBI GenInfo Identifier: | 19856774 |
| NCBI Gene ID: | 7942 |
| NCBI Accession: | P19484.3 |
| UniProt Secondary Accession: | P19484,Q709B3, Q7Z6P9, Q9BRJ5, Q9UJD8, |
| UniProt Related Accession: | P19484 |
| Molecular Weight: | 43,803 Da |
| NCBI Full Name: | Transcription factor EB |
| NCBI Synonym Full Names: | transcription factor EB |
| NCBI Official Symbol: | TFEB |
| NCBI Official Synonym Symbols: | TCFEB; BHLHE35; ALPHATFEB |
| NCBI Protein Information: | transcription factor EB |
| UniProt Protein Name: | Transcription factor EB |
| UniProt Synonym Protein Names: | Class E basic helix-loop-helix protein 35; bHLHe35 |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | TFEB |
| UniProt Entry Name: | TFEB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TFEB antibody (CAB7311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded human colon using TFEB Rabbit pAb (CAB7311) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using TFEB Rabbit pAb (CAB7311) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using TFEB Rabbit pAb (CAB7311) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using TFEB Rabbit pAb (CAB7311) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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