Cell Biology Antibodies 11
Anti-TET3 Antibody (CAB7612)
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- SKU:
- CAB7612
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TET3 Antibody |
| Antibody SKU: | CAB7612 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1525-1660 of human TET3 (NP_001274420.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, HT-29, SKOV3, Mouse lung, Rat thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1525-1660 of human TET3 (NP_001274420.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LAGP SLTE KPWA LGAG DFNS ALKG SPGF QDKL WNPM KGEE GRIP AAGA SQLD RAWQ SFGL PLGS SEKL FGAL KSEE KLWD PFSL EEGP AEEP PSKG AVKE EKGG GGAE EEEE ELWS DSEH NFLD ENIG GVAV APAH |
| Gene ID: | 200424 |
| Uniprot: | O43151 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 77kDa/166kDa/179kDa |
| Observed MW: | 200kDa |
| Synonyms: | TET3, hCG_40738, TET3 |
| Background: |
| UniProt Protein Function: | TET3: Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5- hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Conversion into 5hmC initiates a process leading to cytosine demethylation through deamination into 5-hydroxymethyluracil (5hmU) and subsequent replacement by unmethylated cytosine by the base excision repair system. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin. Belongs to the TET family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 1.14.11.n2; Oxidoreductase Chromosomal Location of Human Ortholog: 2p13.1 Cellular Component: male pronucleus; cytoplasm; female pronucleus Molecular Function:protein binding; DNA binding; metal ion binding Biological Process: protein amino acid O-linked glycosylation; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic |
| NCBI Summary: | Members of the ten-eleven translocation (TET) gene family, including TET3, play a role in the DNA methylation process (Langemeijer et al., 2009 [PubMed 19923888]).[supplied by OMIM, Nov 2010] |
| UniProt Code: | O43151 |
| NCBI GenInfo Identifier: | 190358928 |
| NCBI Gene ID: | 200424 |
| NCBI Accession: | O43151.3 |
| UniProt Secondary Accession: | O43151,Q86Z24, Q8TBM9, A6NEI3, |
| UniProt Related Accession: | O43151 |
| Molecular Weight: | Calculated MW: 77kDa/166kDa/179kDaObserved MW: 200kDa |
| NCBI Full Name: | Methylcytosine dioxygenase TET3 |
| NCBI Synonym Full Names: | tet methylcytosine dioxygenase 3 |
| NCBI Official Symbol: | TET3 |
| NCBI Official Synonym Symbols: | hCG_40738 |
| NCBI Protein Information: | methylcytosine dioxygenase TET3 |
| UniProt Protein Name: | Methylcytosine dioxygenase TET3 |
| Protein Family: | Tetraspanin |
| UniProt Gene Name: | TET3 |
| UniProt Entry Name: | TET3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TET3 antibody (CAB7612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of extracts of rat thymus, using TET3 antibody (CAB7612) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min. |
 | Immunofluorescence analysis of C6 cells using TET3 antibody (CAB7612) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 using TET3 antibody (CAB7612) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U2OS cells using TET3 antibody (CAB7612) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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