Cell Cycle Antibodies 1
Anti-TET2 Antibody (CAB12779)
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- SKU:
- CAB12779
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-TET2 Antibody |
| Antibody SKU: | CAB12779 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1833-2002 of human TET2 (NP_001120680.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:1000 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1833-2002 of human TET2 (NP_001120680.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VQGV ASGA EDND EVWS DSEQ SFLD PDIG GVAV APTH GSIL IECA KREL HATT PLKN PNRN HPTR ISLV FYQH KSMN EPKH GLAL WEAK MAEK AREK EEEC EKYG PDYV PQKS HGKK VKRE PAEP HETS EPTY LRFI KSLA ERTM SVTT DSTV TTSP YAFT RVTG PYNR YI |
| Gene ID: | 54790 |
| Uniprot: | Q6N021 |
| Cellular Location: | |
| Calculated MW: | 130kDa/133kDa/223kDa |
| Observed MW: | 251kDa |
| Synonyms: | MDS, KIAA1546, TET2 |
| Background: | The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Two variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | TET2: Catalyzes the conversion of methylcytosine (5mC) to 5- hydroxymethylcytosine (hmC). Plays an important role in myelopoiesis. The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. TET2 is frequently mutated in myeloproliferative disorders (MPD). These constitute a heterogeneous group of disorders, also known as myeloproliferative diseases or myeloproliferative neoplasms (MPN), characterized by cellular proliferation of one or more hematologic cell lines in the peripheral blood, distinct from acute leukemia. Included diseases are: essential thrombocythemia, polycythemia vera, primary myelofibrosis (chronic idiopathic myelofibrosis). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites. Defects in TET2 are a cause of polycythemia vera (PV). A myeloproliferative disorder characterized by abnormal proliferation of all hematopoietic bone marrow elements, erythroid hyperplasia, an absolute increase in total blood volume, but also by myeloid leukocytosis, thrombocytosis and splenomegaly. TET2 is frequently mutated in systemic mastocytosis; also known as systemic mast cell disease. A condition with features in common with myeloproliferative diseases. It is a clonal disorder of the mast cell and its precursor cells. The clinical symptoms and signs of systemic mastocytosis are due to accumulation of clonally derived mast cells in different tissues, including bone marrow, skin, the gastrointestinal tract, the liver, and the spleen. Defects in TET2 are a cause of myelodysplastic syndrome (MDS). A heterogeneous group of closely related clonal hematopoietic disorders. All are characterized by a hypercellular or hypocellular bone marrow with impaired morphology and maturation, dysplasia of the myeloid, megakaryocytic and/or erythroid lineages, and peripheral blood cytopenias resulting from ineffective blood cell production. Included diseases are: refractory anemia (RA), refractory anemia with ringed sideroblasts (RARS), refractory anemia with excess blasts (RAEB), refractory cytopenia with multilineage dysplasia and ringed sideroblasts (RCMD-RS). Chronic myelomonocytic leukemia (CMML) is a myelodysplastic/myeloproliferative disease. Myelodysplastic syndromes are considered a premalignant condition in a subgroup of patients that often progresses to acute myeloid leukemia (AML). Bone marrow samples from patients display uniformly low levels of hmC in genomic DNA compared to bone marrow samples from healthy controls as well as hypomethylation relative to controls at the majority of differentially methylated CpG sites. Belongs to the TET family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Tumor suppressor; EC 1.14.11.n2; Oxidoreductase Chromosomal Location of Human Ortholog: 4q24 Molecular Function:protein binding; DNA binding; zinc ion binding; ferrous iron binding Biological Process: spleen development; protein amino acid O-linked glycosylation; hemoglobin metabolic process; cell cycle; regulation of gene expression, epigenetic; post-embryonic development; myeloid progenitor cell differentiation; cytosine metabolic process; 5-methylcytosine catabolic process; gene expression; positive regulation of transcription from RNA polymerase II promoter; myeloid cell differentiation; kidney development Disease: Myelodysplastic Syndrome |
| NCBI Summary: | The protein encoded by this gene is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine. The encoded protein is involved in myelopoiesis, and defects in this gene have been associated with several myeloproliferative disorders. Two variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011] |
| UniProt Code: | Q6N021 |
| NCBI GenInfo Identifier: | 239938839 |
| NCBI Gene ID: | 54790 |
| NCBI Accession: | Q6N021.3 |
| UniProt Secondary Accession: | Q6N021,Q2TB88, Q3LIB8, Q96JX5, Q9HCM6, Q9NXW0, B5MDU0 |
| UniProt Related Accession: | Q6N021 |
| Molecular Weight: | 2002 |
| NCBI Full Name: | Methylcytosine dioxygenase TET2 |
| NCBI Synonym Full Names: | tet methylcytosine dioxygenase 2 |
| NCBI Official Symbol: | TET2Â Â |
| NCBI Official Synonym Symbols: | MDS; KIAA1546Â Â |
| NCBI Protein Information: | methylcytosine dioxygenase TET2; tet oncogene family member 2; probable methylcytosine dioxygenase TET2 |
| UniProt Protein Name: | Methylcytosine dioxygenase TET2 |
| Protein Family: | Tetraspanin |
| UniProt Gene Name: | TET2Â Â |
| UniProt Entry Name: | TET2_HUMAN |
 | Western blot analysis of extracts of mouse heart, using TET2 antibody (CAB12779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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