Description
Antibody Name: | Anti-TET1 Antibody (CAB20833) |
Antibody SKU: | CAB20833 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting, Immunohistochemistry |
Reactivity: | Human, Mouse |
Host Species: | Rabbit |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1749-1902 of mouse TET1 (NP_001240786.1). |
Application: | Western blotting, Immunohistochemistry |
Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
Reactivity: | Human, Mouse |
Positive Samples: | ESCS |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1749-1902 of mouse TET1 (NP_001240786.1). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | HNTK SFSS ASST SHLV KDES TDFC PLQA SSAE TSTC TYSK TASG GFAE TSSI LHCT MPSG AHSG ANAA AGEC TGTV QPAE VAAH PHQS LPTA DSPV HAEP LTSP SEQL TSNQ SNQQ LPLL SNSQ KLAS CQVE DERH PEAD EPQH PEDD NLPQ LD |
Cellular Location: | |
Calculated MW: | 219kDa |
Observed MW: | 260KDa |
Synonyms: | LCX, Cxxc6, AA517754, BB001228, D10Ertd17, mKIAA1676, D10Ertd17e, 2510010B09Rik |
Background: |
Immunohistochemistry of paraffin-embedded human testis using TET1 Rabbit mAb at dilution of 1:1600 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Immunohistochemistry of paraffin-embedded mouse testis using TET1 Rabbit mAb at dilution of 1:1600 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. | |
Western blot analysis of extracts of ESCS cells, using TET1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |