Epigenetics & Nuclear Signaling Antibodies 4
Anti-TEFM Antibody (CAB8511)
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- SKU:
- CAB8511
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-TEFM Antibody |
| Antibody SKU: | CAB8511 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 36-210 of human TEFM (NP_078959.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, K-562, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 36-210 of human TEFM (NP_078959.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KSTT PKKI TPNV TFCD ENAK EPEN ALDK LFSS EQQA SILH VLNT ASTK ELEA FRLL RGRR SINI VEHR ENFG PFQN LESL MNVP LFKY KSTV QVCN SILC PKTG REKR KSPE NRFL RKLL KPDI ERER LKAV NSII SIVF GTRR IAWA HLDR KLTV LDWQ QSDR WSLM RGIY SSS |
| Gene ID: | 79736 |
| Uniprot: | Q96QE5 |
| Cellular Location: | Mitochondrion matrix, mitochondrion nucleoid |
| Calculated MW: | 19kDa/41kDa |
| Observed MW: | 37kDa |
| Synonyms: | TEFM, C17orf42 |
| Background: |
| UniProt Protein Function: | Transcription elongation factor which increases mitochondrial RNA polymerase processivity. Regulates transcription of the mitochondrial genome, including genes important for the oxidative phosphorylation machinery. |
| UniProt Code: | Q96QE5 |
| NCBI GenInfo Identifier: | 134053959 |
| NCBI Gene ID: | 79736 |
| NCBI Accession: | NP_078959.3 |
| UniProt Secondary Accession: | Q96QE5,Q6GPG5, Q6PJ19, Q96H04, Q9H5Z9, E1P655, |
| UniProt Related Accession: | Q96QE5 |
| Molecular Weight: | 19,736 Da |
| NCBI Full Name: | transcription elongation factor, mitochondrial |
| NCBI Synonym Full Names: | transcription elongation factor, mitochondrial |
| NCBI Official Symbol: | TEFM |
| NCBI Official Synonym Symbols: | C17orf42 |
| NCBI Protein Information: | transcription elongation factor, mitochondrial |
| UniProt Protein Name: | Transcription elongation factor, mitochondrial |
| Protein Family: | Transcription elongation factor |
| UniProt Gene Name: | TEFM |
 | Western blot analysis of extracts of various cell lines, using TEFM antibody (CAB8511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat lung using TEFM antibody (CAB8511) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using TEFM antibody (CAB8511) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using TEFM antibody (CAB8511) at dilution of 1:100 (40x lens). |
 | Confocal immunofluorescence analysis of U2OS cells using Anti-TEFM Polyclonal Antibody (CAB8511) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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