Epigenetics & Nuclear Signaling Antibodies 3

Anti-TEAD4 Antibody (CAB4151)

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SKU:
CAB4151
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-TEAD4 Antibody
Antibody SKU:CAB4151
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 110-250 of human TEAD4 (NP_003204.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2, K-562, SW620, HeLa, Mouse lung, Mouse skeletal muscle, Mouse liver, Rat lung, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 110-250 of human TEAD4 (NP_003204.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:AREI QAKL KDQA AKDK ALQS MAAM SSAQ IISA TAFH SSMA LARG PGRP AVSG FWQG ALPG QAGT SHDV KPFS QQTY AVQP PLPL PGFE SPAG PAPS PSAP PAPP WQGR SVAS SKLW MLEF SAFL EQQQ DPDT YNKH LFVH I
Gene ID:7004
Uniprot:Q15561
Cellular Location:Nucleus
Calculated MW:34kDa/44kDa/48kDa
Observed MW:48kDa
Synonyms:TEAD4, EFTR-2, RTEF1, TCF13L1, TEF-3, TEF3, TEFR-1, hRTEF-1B
Background:This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene.
UniProt Protein Function:TEAD4: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 12p13.3-p13.2

Cellular Component: nucleoplasm

Molecular Function:protein binding; DNA binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; muscle development; gene expression; skeletal development

NCBI Summary:This gene product is a member of the transcriptional enhancer factor (TEF) family of transcription factors, which contain the TEA/ATTS DNA-binding domain. It is preferentially expressed in the skeletal muscle, and binds to the M-CAT regulatory element found in promoters of muscle-specific genes to direct their gene expression. Alternatively spliced transcripts encoding distinct isoforms, some of which are translated through the use of a non-AUG (UUG) initiation codon, have been described for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q15561
NCBI GenInfo Identifier:215274211
NCBI Gene ID:7004
NCBI Accession:Q15561.3
UniProt Related Accession:Q15561
Molecular Weight:Observed Molecular Weight: (Observed)48kD, 60kD.Predicted Molecular Weight: (Calculated)48kDa.
NCBI Full Name:Transcriptional enhancer factor TEF-3
NCBI Synonym Full Names:TEA domain transcription factor 4
NCBI Official Symbol:TEAD4  
NCBI Official Synonym Symbols:TEF3; RTEF1; TEF-3; EFTR-2; TEFR-1; TCF13L1; hRTEF-1B  
NCBI Protein Information:transcriptional enhancer factor TEF-3
UniProt Protein Name:Transcriptional enhancer factor TEF-3
UniProt Synonym Protein Names:TEA domain family member 4; TEAD-4; Transcription factor 13-like 1; Transcription factor RTEF-1
Protein Family:Transcriptional enhancer factor
UniProt Gene Name:TEAD4  
UniProt Entry Name:TEAD4_HUMAN
Anti-TEAD4 Antibody (CAB4151)Western blot analysis of extracts of various cell lines, using TEAD4 antibody (CAB4151) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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