Antibody Name:	Anti-TEAD1 Antibody
Antibody SKU:	CAB13366
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP ChIP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).

Application:	WB IHC IF IP ChIP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 ChIP 1:20 - 1:50
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, HeLa, Mouse skeletal muscle, Mouse heart, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	AIHN KLGL PGIP RPTF PGAP GFWP GMIQ TGQP GSSQ DVKP FVQQ AYPI QPAV TAPI PGFE PASA PAPS VPAW QGRS IGTT K
Gene ID:	7003
Uniprot:	P28347
Cellular Location:	Nucleus
Calculated MW:	40kDa/47kDa
Observed MW:	50KDa

Synonyms:

TEAD1, AA, NTEF-1, REF1, TCF-13, TCF13, TEAD-1, TEF-1

Background:

This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.

UniProt Protein Function:	TEAD1: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif. Interacts with YAP1 and WWTR1/TAZ. Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 11p15.2 Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm Molecular Function:protein binding; DNA binding; transcription factor activity Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; gene expression Disease: Sveinsson Chorioretinal Atrophy
NCBI Summary:	This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified. [provided by RefSeq, May 2010]
UniProt Code:	P28347
NCBI GenInfo Identifier:	3041733
NCBI Gene ID:	7003
NCBI Accession:	P28347.2
UniProt Secondary Accession:	P28347,A4FUP2, E7EV65,
UniProt Related Accession:	P28347
Molecular Weight:	426
NCBI Full Name:	Transcriptional enhancer factor TEF-1
NCBI Synonym Full Names:	TEA domain family member 1 (SV40 transcriptional enhancer factor)
NCBI Official Symbol:	TEAD1
NCBI Official Synonym Symbols:	AA; REF1; TCF13; TEF-1; NTEF-1; TCF-13; TEAD-1
NCBI Protein Information:	transcriptional enhancer factor TEF-1; protein GT-IIC; transcription factor 13; transcriptional enhancer factor 1
UniProt Protein Name:	Transcriptional enhancer factor TEF-1
UniProt Synonym Protein Names:	NTEF-1; Protein GT-IIC; TEA domain family member 1; TEAD-1; Transcription factor 13; TCF-13
Protein Family:	Transcriptional enhancer factor
UniProt Gene Name:	TEAD1
UniProt Entry Name:	TEAD1_HUMAN

	Western blot analysis of extracts of various cell lines, using TEAD1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
	Western blot analysis of extracts of various cell lines, using TEAD1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded rat brain using TEAD1 Rabbit pAb at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human placenta using TEAD1 Rabbit pAb at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using TEAD1 Rabbit pAb at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using TEAD1 antibody (A13366).
	Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.