Epigenetics & Nuclear Signaling Antibodies 4

Anti-TDP1 Antibody (CAB7984)

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SKU:
CAB7984
Product Type:
Antibody
Applications:
IF
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:TDP1 Rabbit Polyclonal Antibody
Antibody SKU:CAB7984
Antibody Size:20uL, 50uL, 100uL
Application:IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human TDP1 (NP_060789.2).
Application:IF
Recommended Dilution:IF 1:50 - 1:100
Reactivity:Human
Positive Samples:
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human TDP1 (NP_060789.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSQE GDYG RWTI SSSD ESEE EKPK PDKP STSS LLCA RQGA ANEP RYTC SEAQ KAAH KRKI SPVK FSNT DSVL PPKR QKSG SQED LGWC LSSS DDEL QPEM PQKQ AEKV VIKK EKDI SAPN DGTA QRTE NHGA PACH RLKE EEDE YETS GEGQ DIWD MLDK GNPF QFYL TRVS GVKP KYNS GALH IKDI LSPL FGTL VSSA QFNY CFDV DWLV KQYP PEFR KKPI LLVH GDKR EAKA HLHA QAKP YENI SLCQ AKLD IAFG
Gene ID:55775
Uniprot:Q9NUW8
Cellular Location:Cytoplasm, Nucleus
Calculated MW:41kDa/68kDa
Observed MW:
Synonyms:TDP1
Background:The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1).
UniProt Protein Function:TDP1: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. Monomer. Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus. Belongs to the tyrosyl-DNA phosphodiesterase family.
UniProt Protein Details:

Protein type:Phosphodiesterase; EC 3.1.4.-; DNA repair, damage

Chromosomal Location of Human Ortholog: 14q32.11

Cellular Component: cytoplasm; nucleus

Molecular Function:tyrosyl-DNA phosphodiesterase activity; protein binding; double-stranded DNA binding; exonuclease activity; single-stranded DNA binding

Biological Process: single strand break repair; double-strand break repair; DNA repair

Disease: Spinocerebellar Ataxia, Autosomal Recessive, With Axonal Neuropathy

NCBI Summary:The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). [provided by RefSeq, Aug 2016]
UniProt Code:Q9NUW8
NCBI GenInfo Identifier:37999797
NCBI Gene ID:55775
NCBI Accession:Q9NUW8.2
UniProt Related Accession:Q9NUW8
Molecular Weight:Predicted Molecular Mass: 20.4kDaAccurate Molecular Mass: 25kDa
NCBI Full Name:Tyrosyl-DNA phosphodiesterase 1
NCBI Synonym Full Names:tyrosyl-DNA phosphodiesterase 1
NCBI Official Symbol:TDP1  
NCBI Protein Information:tyrosyl-DNA phosphodiesterase 1
UniProt Protein Name:Tyrosyl-DNA phosphodiesterase 1
Protein Family:Tyrosyl-DNA phosphodiesterase
UniProt Gene Name:TDP1  
UniProt Entry Name:TYDP1_HUMAN
Anti-TDP1 Polyclonal Antibody (CAB7984)Immunofluorescence analysis of A549 cells using TDP1 antibody (CAB7984). Blue: DAPI for nuclear staining.
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