Epigenetics & Nuclear Signaling Antibodies 2

Anti-TDG Antibody (CAB5756)

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SKU:
CAB5756
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-TDG Antibody
Antibody SKU:CAB5756
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human TDG (NP_003202.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, MCF7, HL-60, Mouse stomach, rat eye, rat stomach
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human TDG (NP_003202.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEAE NAGS YSLQ QAQA FYTF PFQQ LMAE APNM AVVN EQQM PEEV PAPA PAQE PVQE APKG RKRK PRTT EPKQ PVEP KKPV ESKK SGKS AKSK EKQE KITD TFKV KRKV DRFN GVSE AELL TKTL PDIL TFNL DIVI IGIN PGLM AAYK GHHY PGPG NHFW
Gene ID:6996
Uniprot:Q13569
Cellular Location:Nucleus
Calculated MW:46kDa
Observed MW:46kDa/92kDa
Synonyms:TDG, hTDG
Background:The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.
UniProt Protein Function:TDG: In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar- phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. Belongs to the TDG/mug DNA glycosylase family.
UniProt Protein Details:

Protein type:DNA repair, damage; EC 3.2.2.29; Nuclear receptor co-regulator; Hydrolase

Chromosomal Location of Human Ortholog: 12q24.1

Cellular Component: nucleoplasm; PML body; plasma membrane; nucleus

Molecular Function:protein domain specific binding; protein binding; protein homodimerization activity; structure-specific DNA binding; SUMO binding; protein kinase C binding; DNA N-glycosylase activity; double-stranded DNA binding; damaged DNA binding; transcription factor binding; pyrimidine-specific mismatch base pair DNA N-glycosylase activity; mismatched DNA binding

Biological Process: transcription from RNA polymerase II promoter; embryonic development; mismatch repair; base-excision repair, AP site formation; depyrimidination; base-excision repair; gene expression; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; DNA repair; negative regulation of protein binding; regulation of gene expression, epigenetic

NCBI Summary:The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008]
UniProt Code:Q13569
NCBI GenInfo Identifier:46397791
NCBI Gene ID:6996
NCBI Accession:Q13569.2
UniProt Secondary Accession:Q13569,Q8IUZ6, Q8IZM3,
UniProt Related Accession:Q13569
Molecular Weight:410
NCBI Full Name:G/T mismatch-specific thymine DNA glycosylase
NCBI Synonym Full Names:thymine-DNA glycosylase
NCBI Official Symbol:TDG  
NCBI Official Synonym Symbols:hTDG  
NCBI Protein Information:G/T mismatch-specific thymine DNA glycosylase
UniProt Protein Name:G/T mismatch-specific thymine DNA glycosylase
UniProt Synonym Protein Names:Thymine-DNA glycosylase; hTDG
Protein Family:G/T mismatch-specific thymine DNA glycosylase
UniProt Gene Name:TDG  
UniProt Entry Name:TDG_HUMAN
Anti-TDG Antibody (CAB5756)Western blot analysis of extracts of various cell lines, using TDG antibody (CAB5756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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