Antibody Name:	Anti-TCIRG1 Antibody
Antibody SKU:	CAB15382
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human TCIRG1 (NP_006010.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U2OS, A-549, BxPC-3, U-937, HL-60, Mouse thymus, Mouse skeletal muscle, Rat thymus, Rat skeletal muscle

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human TCIRG1 (NP_006010.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGSM FRSE EVAL VQLF LPTA AAYT CVSR LGEL GLVE FRDL NASV SAFQ RRFV VDVR RCEE LEKT FTFL QEEV RRAG LVLP PPKG RLPA PPPR DLLR IQEE TERL AQEL RDVR GNQQ ALRA QLHQ LQLH AA
Gene ID:	10312
Uniprot:	Q13488
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	68kDa/92kDa
Observed MW:	93kDa

Synonyms:

TCIRG1, ATP6N1C, ATP6V0A3, Atp6i, OC-116kDa, OC116, OPTB1, Stv1, TIRC7, Vph1, a3

Background:

This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis.

UniProt Protein Function:	Part of the proton channel of V-ATPases. Seems to be directly involved in T-cell activation.
NCBI Summary:	This gene encodes a subunit of a large protein complex known as a vacuolar H+-ATPase (V-ATPase). The protein complex acts as a pump to move protons across the membrane. This movement of protons helps regulate the pH of cells and their surrounding environment. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. V-ATPase is comprised of a cytosolic V1 domain and a transmembrane V0 domain. Alternative splicing results in multiple transcript variants. Mutations in this gene are associated with infantile malignant osteopetrosis. [provided by RefSeq, May 2017]
UniProt Code:	Q13488
NCBI GenInfo Identifier:	19924145
NCBI Gene ID:	10312
NCBI Accession:	NP_006010.2
UniProt Secondary Accession:	Q13488,O75877, Q8WVC5,
UniProt Related Accession:	Q13488
Molecular Weight:	Calculated: 68kDa; 92kDaObserved: 93kDa
NCBI Full Name:	V-type proton ATPase 116 kDa subunit a isoform 3 isoform a
NCBI Synonym Full Names:	T cell immune regulator 1, ATPase H+ transporting V0 subunit a3
NCBI Official Symbol:	TCIRG1
NCBI Official Synonym Symbols:	a3; Stv1; Vph1; Atp6i; OC116; OPTB1; TIRC7; ATP6N1C; ATP6V0A3; OC-116kDa
NCBI Protein Information:	V-type proton ATPase 116 kDa subunit a isoform 3; V-type proton ATPase 116 kDa subunit a
UniProt Protein Name:	V-type proton ATPase 116 kDa subunit a isoform 3
UniProt Synonym Protein Names:	Osteoclastic proton pump 116 kDa subunit; OC-116 kDa; OC116; T-cell immune regulator 1; T-cell immune response cDNA7 protein; TIRC7; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 3
Protein Family:	V-type proton ATPase
UniProt Gene Name:	TCIRG1

	Western blot analysis of extracts of various cell lines, using TCIRG1 antibody (CAB15382) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded Mouse spleen using TCIRG1 Rabbit pAb (CAB15382) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat ovary using TCIRG1 Rabbit pAb (CAB15382) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human liver using TCIRG1 Rabbit pAb (CAB15382) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using TCIRG1 Polyclonal Antibody (CAB15382) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.