Epigenetics & Nuclear Signaling Antibodies 3
Anti-TCF7 Antibody (CAB3091)
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- SKU:
- CAB3091
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-TCF7 Antibody |
| Antibody SKU: | CAB3091 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human TCF7 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse spleen |
| Immunogen: | A synthetic peptide of human TCF7 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 6932 |
| Uniprot: | P36402 |
| Cellular Location: | Nucleus |
| Calculated MW: | 28-42kDa/53-54kDa |
| Observed MW: | 53kDa |
| Synonyms: | TCF7, TCF-1 |
| Background: | The protein encoded by this gene is a transcriptional activator that plays an important role in lymphocyte differentiation. This gene is expressed predominantly in T-cells. The encoded protein can bind an enhancer element and activate the CD3E gene, and it also may repress the CTNNB1 and TCF7L2 genes through a feedback mechanism. Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | TCF7: Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T- lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1. Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with AES, TLE1, TLE2, TLE3 and TLE4. By TCF7L2 and CTNNB1. Predominantly expressed in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. Belongs to the TCF/LEF family. 16 isoforms of the human protein are produced by alternative promoter. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: nucleoplasm; transcription factor complex; nucleus Molecular Function:protein binding; sequence-specific DNA binding; beta-catenin binding; chromatin binding; transcription factor activity Biological Process: neural tube development; transcription, DNA-dependent; alpha-beta T cell differentiation; Wnt receptor signaling pathway through beta-catenin; regulation of cell proliferation; regulation of transcription from RNA polymerase II promoter; embryonic digestive tract morphogenesis; generation of neurons; T cell receptor V(D)J recombination; immune response; brain development; embryonic genitalia morphogenesis; embryonic hindgut morphogenesis |
| NCBI Summary: | The protein encoded by this gene is a transcriptional activator that plays an important role in lymphocyte differentiation. This gene is expressed predominantly in T-cells. The encoded protein can bind an enhancer element and activate the CD3E gene, and it also may repress the CTNNB1 and TCF7L2 genes through a feedback mechanism. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| UniProt Code: | P36402 |
| NCBI GenInfo Identifier: | 209572716 |
| NCBI Gene ID: | 6932 |
| NCBI Accession: | P36402.3 |
| UniProt Secondary Accession: | P36402,Q86WR9, Q9UKI4, B3KSH3, |
| UniProt Related Accession: | P36402 |
| Molecular Weight: | 384 |
| NCBI Full Name: | Transcription factor 7 |
| NCBI Synonym Full Names: | transcription factor 7 (T-cell specific, HMG-box) |
| NCBI Official Symbol: | TCF7 |
| NCBI Official Synonym Symbols: | TCF-1 |
| NCBI Protein Information: | transcription factor 7; T-cell-specific transcription factor 1 |
| UniProt Protein Name: | Transcription factor 7 |
| UniProt Synonym Protein Names: | T-cell-specific transcription factor 1; T-cell factor 1; TCF-1 |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | TCF7 |
| UniProt Entry Name: | TCF7_HUMAN |
 | Western blot analysis of extracts of mouse spleen, using TCF7 antibody (CAB3091) at 1:300 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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