Anti-TCF4/TCF7L2 Antibody (CAB20770)

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SKU:
CAB20770

Description

Antibody Name:Anti-TCF4/TCF7L2 Antibody (CAB20770)
Antibody SKU:CAB20770
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 124-188 of human TCF4/TCF7L2 (NP_001354872.1).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:SW620, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 124-188 of human TCF4/TCF7L2 (NP_001354872.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TART LHFQ SGST HYSA YKTI EHQI AVQY LQMK WPLL DVQA GSLQ SRQA LKDA RSPS PAHI VSNK V
Cellular Location:
Calculated MW:68kDa
Observed MW:60KDa/75KDa
Synonyms:TCF-4, TCF4, TCF7L2
Background:This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.provided by RefSeq, Oct 2010
Western blot analysis of extracts of various cell lines, using TCF4/TCF7L2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Western blot analysis of extracts of various cell lines, using TCF4/TCF7L2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
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Additional Information

Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Goat
Isotype:
Rhodamine conjugated IgG
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