Description
Antibody Name: | Anti-TCF4/TCF7L2 Antibody (CAB20770) |
Antibody SKU: | CAB20770 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Human, Mouse |
Host Species: | Rabbit |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 124-188 of human TCF4/TCF7L2 (NP_001354872.1). |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human, Mouse |
Positive Samples: | SW620, Mouse brain |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 124-188 of human TCF4/TCF7L2 (NP_001354872.1). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | TART LHFQ SGST HYSA YKTI EHQI AVQY LQMK WPLL DVQA GSLQ SRQA LKDA RSPS PAHI VSNK V |
Cellular Location: | |
Calculated MW: | 68kDa |
Observed MW: | 60KDa/75KDa |
Synonyms: | TCF-4, TCF4, TCF7L2 |
Background: | This gene encodes a high mobility group (HMG) box-containing transcription factor that plays a key role in the Wnt signaling pathway. The protein has been implicated in blood glucose homeostasis. Genetic variants of this gene are associated with increased risk of type 2 diabetes. Several transcript variants encoding multiple different isoforms have been found for this gene.provided by RefSeq, Oct 2010 |
Western blot analysis of extracts of various cell lines, using TCF4/TCF7L2 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |