Antibody Name:	Anti-TCEB3 Antibody
Antibody SKU:	CAB6994
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 679-798 of human TCEB3 (NP_003189.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, HeLa, Raji, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 679-798 of human TCEB3 (NP_003189.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ANKP KGRQ AKMA FVNS VAKP PRDV RRRQ EKFG TGGA AVPE KIKI KPAP YPMG SSHA SASS ISFN PSPE EPAY DGPS TSSA HLAP VVSS TVSY DPRK PTVK KIAP MMAK TIKA FKNR FSRR
Gene ID:	6924
Uniprot:	Q14241
Cellular Location:	Nucleus
Calculated MW:	89kDa
Observed MW:	105kDa

Synonyms:

ELOA, SIII, SIII p110, TCEB3, TCEB3A, SIIIp110, elongin-A

Background:

This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.

UniProt Protein Function:	TCEB3: SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). Heterotrimer of an A (A1, A2 or A3), B and C subunit. The C subunit mediates the binding of the elongin BC complex to subunit A.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 1p36.1 Cellular Component: nucleoplasm; extracellular space; cytoplasm; integral to membrane; nucleus Molecular Function:DNA binding Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; gene expression
NCBI Summary:	This gene encodes the protein elongin A, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. [provided by RefSeq, Jul 2008]
UniProt Code:	Q14241
NCBI GenInfo Identifier:	294862430
NCBI Gene ID:	6924
NCBI Accession:	Q14241.2
UniProt Related Accession:	Q14241
Molecular Weight:	~ 105kDa
NCBI Full Name:	Elongin-A
NCBI Synonym Full Names:	elongin A
NCBI Official Symbol:	ELOA
NCBI Official Synonym Symbols:	SIII; TCEB3; TCEB3A; SIII p110
NCBI Protein Information:	elongin-A
UniProt Protein Name:	Transcription elongation factor B polypeptide 3
UniProt Synonym Protein Names:	Elongin 110 kDa subunit; Elongin-A; EloA; RNA polymerase II transcription factor SIII subunit A1; SIII p110
Protein Family:	Protein
UniProt Gene Name:	TCEB3
UniProt Entry Name:	ELOA1_HUMAN

	Western blot analysis of extracts of various cell lines, using TCEB3 antibody (CAB6994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human kidney cancer using TCEB3 antibody (CAB6994) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF7 cells using TCEB3 antibody (CAB6994).