Name: Anti-TCEA1 Antibody (CAB20972)
SKU: CAB20972
Availability: OutOfStock

Antibody Name:	Anti-TCEA1 Antibody (CAB20972)
Antibody SKU:	CAB20972
Antibody Size:	50µL, 100µL
Application:	Western blotting, Immunohistochemistry, Immunofluorescence
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of Human TCEA1.

Application:	Western blotting, Immunohistochemistry, Immunofluorescence
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SP2/0, HeLa, K-562, Mouse liver, Mouse brain, Rat thymus

Immunogen:	Recombinant protein of Human TCEA1.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Cellular Location:	Nucleus
Calculated MW:	31kDa/33kDa
Observed MW:	40KDa

Synonyms:	TCEA1, GTF2S, SII, TCEA, TF2S, TFIIS
Background:

	Immunofluorescence analysis of HeLa cells using TCEA1 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using TCEA1 Rabbit mAb at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.
	Immunohistochemistry of paraffin-embedded rat ovary using TCEA1 Rabbit mAb at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded mouse spleen using TCEA1 Rabbit mAb at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded mouse liver using TCEA1 Rabbit mAb at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using TCEA1 Rabbit mAb at dilution of 1:25 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol.
	Western blot analysis of extracts of various cell lines, using TCEA1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.