Epigenetics & Nuclear Signaling Antibodies 2
Anti-TBX5 Antibody (CAB15590)
- SKU:
- CAB15590
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-TBX5 Antibody |
| Antibody SKU: | CAB15590 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human TBX5 (NP_852259.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human TBX5 (NP_852259.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MADA DEGF GLAH TPLE PDAK DLPC DSKP ESAL GAPS KSPS SPQA AFTQ QGME GIKV FLHE RELW LKFH EV |
| Gene ID: | 6910 |
| Uniprot: | Q99593 |
| Cellular Location: | Nucleus |
| Calculated MW: | 39kDa/52kDa/57kDa |
| Observed MW: | 58kDa |
| Synonyms: | TBX5, HOS, T-box 5 |
| Background: | This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. |
| UniProt Protein Function: | TBX5: Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation. Defects in TBX5 are the cause of Holt-Oram syndrome (HOS). HOS is a developmental disorder affecting the heart and upper limbs. It is characterized by thumb anomaly and atrial septal defects. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 12q24.1 Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus Molecular Function:protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity; transcription factor binding Biological Process: embryonic forelimb morphogenesis; pericardium development; transcription initiation from RNA polymerase II promoter; heart development; positive regulation of transcription, DNA-dependent; pattern specification process; cardiac muscle cell differentiation; forelimb morphogenesis; negative regulation of cell proliferation; cell-cell signaling; apoptotic nuclear changes; negative regulation of cardiac muscle cell proliferation; positive regulation of cardioblast differentiation; morphogenesis of an epithelium; gene expression; positive regulation of transcription from RNA polymerase II promoter; negative regulation of cell migration; lung development; embryonic limb morphogenesis; positive regulation of cardiac muscle cell proliferation Disease: Holt-oram Syndrome |
| NCBI Summary: | This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is closely linked to related family member T-box 3 (ulnar mammary syndrome) on human chromosome 12. The encoded protein may play a role in heart development and specification of limb identity. Mutations in this gene have been associated with Holt-Oram syndrome, a developmental disorder affecting the heart and upper limbs. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99593 |
| NCBI GenInfo Identifier: | 12644474 |
| NCBI Gene ID: | 6910 |
| NCBI Accession: | Q99593.2 |
| UniProt Related Accession: | Q99593 |
| Molecular Weight: | |
| NCBI Full Name: | T-box transcription factor TBX5 |
| NCBI Synonym Full Names: | T-box transcription factor 5 |
| NCBI Official Symbol: | TBX5 |
| NCBI Official Synonym Symbols: | HOS |
| NCBI Protein Information: | T-box transcription factor TBX5 |
| UniProt Protein Name: | T-box transcription factor TBX5 |
| Protein Family: | T-box transcription factor |
| UniProt Gene Name: | TBX5 |
| UniProt Entry Name: | TBX5_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TBX5 antibody (CAB15590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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