Cell Biology Antibodies 11

Anti-TBCE Antibody (CAB7453)

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SKU:
CAB7453
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TBCE Antibody
Antibody SKU:CAB7453
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 328-527 of human TBCE (NP_003184.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:A-549, SW620, NCI-H460, BT-474
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 328-527 of human TBCE (NP_003184.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LEKL PSLR ALSC LRNP LTKE DKEA ETAR LLII ASIG QLKT LNKC EILP EERR RAEL DYRK AFGN EWKQ AGGH KDPE KNRL SEEF LTAH PRYQ FLCL KYGA PEDW ELKT QQPL MLKN QLLT LKIK YPHQ LDQK VLEK QLPG SMTI QKVK GLLS RLLK VPVS DLLL SYES PKKP GREI ELEN DLKS LQFY SVEN GDCL LVRW
Gene ID:6905
Uniprot:Q15813
Cellular Location:Cytoplasm, cytoskeleton
Calculated MW:59kDa/64kDa
Observed MW:59kDa
Synonyms:TBCE, HRD, KCS, KCS1, PEAMO, pac2
Background:Cofactor E is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. Two transcript variants encoding the same protein have been found for this gene.
UniProt Protein Function:TBCE: Tubulin-folding protein; involved in the second step of the tubulin folding pathway. Seems to be implicated in the maintenance of the neuronal microtubule network. Involved in regulation of tubulin heterodimer dissociation. Defects in TBCE are a cause of hypoparathyroidism- retardation-dysmorphism syndrome (HRD); also known as hypoparathyroidism with short stature, mental retardation, and seizures or Sanjad-Sakati syndrome. HRD is an autosomal recessive disorder reported almost exclusively in Middle Eastern populations. Defects in TBCE are the cause of Kenny-Caffey syndrome type 1 (KCS1). KCS1 is similar to HRD with the additional features of osteosclerosis and recurrent bacterial infections. Belongs to the TBCE family.
UniProt Protein Details:

Protein type:Chaperone

Chromosomal Location of Human Ortholog: 1q42.3

Cellular Component: microtubule; cytoplasm

Molecular Function:chaperone binding

Biological Process: 'de novo' posttranslational protein folding; developmental growth; cellular protein metabolic process; protein folding; adult locomotory behavior; muscle atrophy; microtubule cytoskeleton organization and biogenesis; peripheral nervous system neuron axonogenesis; post-chaperonin tubulin folding pathway; post-embryonic development

Disease: Hypoparathyroidism-retardation-dysmorphism Syndrome; Kenny-caffey Syndrome, Type 1

NCBI Summary:Cofactor E is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q15813
NCBI GenInfo Identifier:74762146
NCBI Gene ID:6905
NCBI Accession:Q15813.1
UniProt Secondary Accession:Q15813,A8K8C2, B7Z3P1,
UniProt Related Accession:Q15813
Molecular Weight:527
NCBI Full Name:Tubulin-specific chaperone E
NCBI Synonym Full Names:tubulin folding cofactor E
NCBI Official Symbol:TBCE  
NCBI Official Synonym Symbols:HRD; KCS; KCS1; pac2  
NCBI Protein Information:tubulin-specific chaperone E; hypoparathyroidism, growth and mental retardation, and dysmorphism
UniProt Protein Name:Tubulin-specific chaperone E
UniProt Synonym Protein Names:Tubulin-folding cofactor E
Protein Family:Tubulin-specific chaperone
UniProt Gene Name:TBCE  
UniProt Entry Name:TBCE_HUMAN
Anti-TBCE Antibody (CAB7453)Western blot analysis of extracts of various cell lines, using TBCE antibody (CAB7453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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