Cell Biology Antibodies 3

Anti-TBCA Antibody (CAB13050)

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SKU:
CAB13050
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-TBCA Antibody
Antibody SKU:CAB13050
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human TBCA (NP_004598.1).
Application:WB IHC
Recommended Dilution:WB 1:1000 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:OVCAR3, HeLa, 293T, U-87MG, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human TBCA (NP_004598.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MADP RVRQ IKIK TGVV KRLV KEKV MYEK EAKQ QEEK IEKM RAED GENY DIKK QAEI LQES RMMI PDCQ RRLE AAYL DLQR ILEN EKDL EEAE EYKE ARLV LDSV KLEA
Gene ID:6902
Uniprot:O75347
Cellular Location:Cytoplasm, cytoskeleton
Calculated MW:12kDa/15kDa
Observed MW:13kDa
Synonyms:TBCA
Background:The product of this gene is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. This gene encodes chaperonin cofactor A. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:TBCA: Tubulin-folding protein; involved in the early step of the tubulin folding pathway. Belongs to the TBCA family.
UniProt Protein Details:

Protein type:Chaperone

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: microtubule cytoskeleton; microtubule; cytoplasm; nucleolus

Molecular Function:chaperone binding; unfolded protein binding

Biological Process: tubulin folding; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; post-chaperonin tubulin folding pathway

NCBI Summary:The product of this gene is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. This gene encodes chaperonin cofactor A. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
UniProt Code:O75347
NCBI GenInfo Identifier:6831691
NCBI Gene ID:6902
NCBI Accession:O75347.3
UniProt Related Accession:O75347
Molecular Weight:12,855 Da
NCBI Full Name:Tubulin-specific chaperone A
NCBI Synonym Full Names:tubulin folding cofactor A
NCBI Official Symbol:TBCA  
NCBI Protein Information:tubulin-specific chaperone A; CFA; chaperonin cofactor A; TCP1-chaperonin cofactor A; tubulin-folding cofactor A
UniProt Protein Name:Tubulin-specific chaperone A
UniProt Synonym Protein Names:TCP1-chaperonin cofactor A; Tubulin-folding cofactor A; CFA
UniProt Gene Name:TBCA  
UniProt Entry Name:TBCA_HUMAN
Anti-TBCA Antibody (CAB13050)Western blot analysis of extracts of various cell lines, using TBCA antibody (CAB13050) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-TBCA Antibody (CAB13050)Immunohistochemistry of paraffin-embedded rat brain using TBCA antibody (CAB13050) at dilution of 1:100 (40x lens).
Anti-TBCA Antibody (CAB13050)Immunohistochemistry of paraffin-embedded mouse spinal cord using TBCA antibody (CAB13050) at dilution of 1:100 (40x lens).
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