Metabolism Antibodies 2

Anti-TAT Antibody (CAB6764)

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SKU:
CAB6764
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-TAT Antibody
Antibody SKU:CAB6764
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TAT (NP_000344.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:BT-474, Raji, A673, HepG2, Mouse liver, Mouse kidney, Mouse heart, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TAT (NP_000344.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDPY MIQM SSKG NLPS ILDV HVNV GGRS SVPG KMKG RKAR WSVR PSDM AKKT FNPI RAIV DNMK VKPN PNKT MISL SIGD PTVF GNLP TDPE VTQA MKDA LDSG KYNG YAPS IGFL SSRE EIAS YYHC PEAP LEAK DVIL TSGC SQAI DLCL AVLA NPGQ NILV PRPG FSLY KTLA ESMG IEVK LYNL LPEK SWEI DLKQ LEYL IDEK TACL IVNN PSNP CGSV FSKR HLQK ILAV AARQ CVPI LADE IYGD MVFS DCKY EPLA TLST DV
Gene ID:6898
Uniprot:P17735
Cellular Location:
Calculated MW:50kDa
Observed MW:55kDa
Synonyms:TAT
Background:This nuclear gene encodes a mitochondrial protein tyrosine aminotransferase which is present in the liver and catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Mutations in this gene cause tyrosinemia (type II, Richner-Hanhart syndrome), a disorder accompanied by major skin and corneal lesions, with possible mental retardation. A regulator gene for tyrosine aminotransferase is X-linked.
UniProt Protein Function:TAT: Transaminase involved in tyrosine breakdown. Converts tyrosine to p-hydroxyphenylpyruvate. Can catalyze the reverse reaction, using glutamic acid, with 2-oxoglutarate as cosubstrate (in vitro). Has much lower affinity and transaminase activity towards phenylalanine. Defects in TAT are the cause of tyrosinemia type 2 (TYRO2); also known as Richner-Hanhart syndrome. TYRO2 is an inborn error of metabolism characterized by elevations of tyrosine in the blood and urine, and oculocutaneous manifestations. Typical features include palmoplantar keratosis, painful corneal ulcers, and mental retardation. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
UniProt Protein Details:

Protein type:Cofactor and Vitamin Metabolism - ubiquinone and other terpenoid-quinone biosynthesis; Mitochondrial; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine; EC 2.6.1.5; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - cysteine and methionine; Transferase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: mitochondrion; cytosol

Molecular Function:amino acid binding; tyrosine transaminase activity; pyridoxal phosphate binding

Biological Process: response to mercury ion; L-phenylalanine catabolic process; glutamate metabolic process; tyrosine catabolic process; response to glucocorticoid stimulus; biosynthetic process; response to oxidative stress; 2-oxoglutarate metabolic process

Disease: Tyrosinemia, Type Ii

NCBI Summary:This nuclear gene encodes a mitochondrial protein tyrosine aminotransferase which is present in the liver and catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Mutations in this gene cause tyrosinemia (type II, Richner-Hanhart syndrome), a disorder accompanied by major skin and corneal lesions, with possible mental retardation. A regulator gene for tyrosine aminotransferase is X-linked. [provided by RefSeq, Jul 2008]
UniProt Code:P17735
NCBI GenInfo Identifier:114713
NCBI Gene ID:6898
NCBI Accession:P17735.1
UniProt Secondary Accession:P17735,B2R8I1, D3DWS2,
UniProt Related Accession:P17735
Molecular Weight:454
NCBI Full Name:Tyrosine aminotransferase
NCBI Synonym Full Names:tyrosine aminotransferase
NCBI Official Symbol:TAT  
NCBI Protein Information:tyrosine aminotransferase; tyrosine aminotransferase, cytosolic; L-tyrosine:2-oxoglutarate aminotransferase
UniProt Protein Name:Tyrosine aminotransferase
UniProt Synonym Protein Names:L-tyrosine:2-oxoglutarate aminotransferase
Protein Family:Tat-binding
UniProt Gene Name:TAT  
UniProt Entry Name:ATTY_HUMAN
Anti-TAT Antibody (CAB6764)Western blot analysis of extracts of various cell lines, using TAT antibody (CAB6764) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5min.
Anti-TAT Antibody (CAB6764)Immunofluorescence analysis of MCF-7 cells using TAT antibody (CAB6764). Blue: DAPI for nuclear staining.
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