Cell Biology Antibodies 1
Anti-TARDBP Antibody (CAB0538)
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- SKU:
- CAB0538
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TARDBP Antibody |
| Antibody SKU: | CAB0538 |
| Antibody Size: | 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human TARDBP |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, K-562, Raji, Mouse brain |
| Immunogen: | A synthetic peptide of human TARDBP |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 23435 |
| Uniprot: | Q13148 |
| Cellular Location: | Nucleus |
| Calculated MW: | 31kDa/44kDa |
| Observed MW: | 43kDa |
| Synonyms: | ALS10, TDP-43, TARDBP, TDP43 |
| Background: | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. |
| NCBI Summary: | HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13148 |
| NCBI GenInfo Identifier: | 20140568 |
| NCBI Gene ID: | 23435 |
| NCBI Accession: | |
| UniProt Related Accession: | Q13148 |
| Molecular Weight: | 44 |
| NCBI Full Name: | TAR DNA-binding protein 43 |
| NCBI Synonym Full Names: | TAR DNA binding protein |
| NCBI Official Symbol: | TARDBP |
| NCBI Official Synonym Symbols: | ALS10; TDP-43 |
| NCBI Protein Information: | TAR DNA-binding protein 43 |
| UniProt Protein Name: | |
| UniProt Gene Name: |
 | Western blot analysis of extracts of various cell lines, using TARDBP antibody (CAB0538) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat heart using TARDBP antibody (CAB0538) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human breast cancer using TARDBP antibody (CAB0538) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse brain using TARDBP antibody (CAB0538) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using TARDBP antibody (CAB0538) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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