Antibody Name:	Anti-TAP1 Antibody
Antibody SKU:	CAB6213
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 524-808 of human TAP1 (NP_000584.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	SKOV3, Jurkat, Mouse lung, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 524-808 of human TAP1 (NP_000584.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IYPR VQKA VGSS EKIF EYLD RTPR CPPS GLLT PLHL EGLV QFQD VSFA YPNR PDVL VLQG LTFT LRPG EVTA LVGP NGSG KSTV AALL QNLY QPTG GQLL LDGK PLPQ YEHR YLHR QVAA VGQE PQVF GRSL QENI AYGL TQKP TMEE ITAA AVKS GAHS FISG LPQG YDTE VDEA GSQL SGGQ RQAV ALAR ALIR KPCV LILD DATS ALDA NSQL QVEQ LLYE SPER YSRS VLLI TQHL SLVE QADH ILFL EGGA IREG GTHQ QLME KKGC YWAM VQAP ADAP E
Gene ID:	6890
Uniprot:	Q03518
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	87kDa
Observed MW:	68kDa

Synonyms:

TAP1, ABC17, ABCB2, APT1, D6S114E, PSF-1, PSF1, RING4, TAP1*0102N, TAP1N

Background:

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
NCBI Summary:	The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014]
UniProt Code:	Q03518
NCBI GenInfo Identifier:	215273957
NCBI Gene ID:	6890
NCBI Accession:	Q03518.2
UniProt Secondary Accession:	Q03518,Q16149, Q96CP4,
UniProt Related Accession:	Q03518
Molecular Weight:	87,218 Da
NCBI Full Name:	Antigen peptide transporter 1
NCBI Synonym Full Names:	transporter 1, ATP binding cassette subfamily B member
NCBI Official Symbol:	TAP1
NCBI Official Synonym Symbols:	APT1; PSF1; ABC17; ABCB2; PSF-1; RING4; TAP1N; D6S114E; TAP1*0102N
NCBI Protein Information:	antigen peptide transporter 1
UniProt Protein Name:	Antigen peptide transporter 1
UniProt Synonym Protein Names:	ATP-binding cassette sub-family B member 2; Peptide supply factor 1; Peptide transporter PSF1; PSF-1; Peptide transporter TAP1; Peptide transporter involved in antigen processing 1; Really interesting new gene 4 protein
Protein Family:	Protein
UniProt Gene Name:	TAP1
UniProt Entry Name:	TAP1_HUMAN

	Western blot analysis of extracts of various cell lines, using TAP1 antibody (CAB6213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of extracts of various cell lines, using TAP1 antibody (CAB6213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.