Antibody Name:	Anti-TAF9 Antibody
Antibody SKU:	CAB2021
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human TAF9 (NP_057367.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	BT-474, HeLa, K-562

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human TAF9 (NP_057367.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MLLP NILL TGTP GVGK TTLG KELA SKSG LKYI NVGD LARE EQLY DGYD EEYD CPIL DEDR VVDE LDNQ MREG GVIV DYHG CDFF PERW FHIV FVLR TDTN VLYE RLET RGYN EKKL TDNI QCEI FQVL YEEA TASY KEEI VHQL PSNK PEEL ENNV DQIL KWIE QWIK DHNS
Gene ID:	6880
Uniprot:	Q16594
Cellular Location:	Nucleus
Calculated MW:	28kDa
Observed MW:	20kDa

Synonyms:

TAF9, MGC:5067, STAF31/32, TAF2G, TAFII-31, TAFII-32, TAFII31, TAFII32, TAFIID32

Background:

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. In human, TAF9 and AK6 (GeneID: 102157402) are two distinct genes that share 5' exons. A similar but distinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified on chromosome 19. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	TAFII31: a component of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is essential for mediating regulation of RNA polymerase transcription. TAFII31 binds to p53, the basal transcription factor GTF2B, and the acidic transactivator viral protein 16 (VP16). It is a coactivator for P53.
UniProt Protein Details:	Protein type:EC 2.7.4.3; Transcription initiation complex; DNA-binding; Acetyltransferase; Kinase, other Chromosomal Location of Human Ortholog: 5q13.2 Cellular Component: nucleoplasm; PCAF complex; transcription factor TFIID complex Molecular Function:protein binding; histone acetyltransferase activity; DNA binding; transcription activator binding; protein heterodimerization activity; p53 binding; transcription coactivator activity Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; viral reproduction; protein stabilization; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell growth; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; response to DNA damage stimulus; negative regulation of apoptosis
NCBI Summary:	Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. In human, TAF9 and AK6 (GeneID: 102157402) are two distinct genes that share 5' exons. A similar but distinct gene (TAF9L) has been found on the X chromosome and a pseudogene has been identified on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
UniProt Code:	Q16594
NCBI GenInfo Identifier:	2498981
NCBI Gene ID:	6880
NCBI Accession:	Q16594.1
UniProt Related Accession:	Q16594
Molecular Weight:
NCBI Full Name:	Transcription initiation factor TFIID subunit 9
NCBI Synonym Full Names:	TATA-box binding protein associated factor 9
NCBI Official Symbol:	TAF9
NCBI Official Synonym Symbols:	TAF2G; TAFII31; TAFII32; MGC:5067; TAFII-31; TAFII-32; TAFIID32; STAF31/32
NCBI Protein Information:	transcription initiation factor TFIID subunit 9
UniProt Protein Name:	Transcription initiation factor TFIID subunit 9
UniProt Synonym Protein Names:	RNA polymerase II TBP-associated factor subunit G; STAF31/32; Transcription initiation factor TFIID 31 kDa subunit; TAFII-31; TAFII31; Transcription initiation factor TFIID 32 kDa subunit; TAFII-32; TAFII32
Protein Family:	Transcription initiation factor
UniProt Gene Name:	TAF9
UniProt Entry Name:	TAF9_HUMAN

	Western blot analysis of extracts of various cell lines, using TAF9 antibody (CAB2021) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded rat heart using TAF9 antibody (CAB2021) at dilution of 1:100 (40x lens).