Antibody Name:	Anti-TAF5 Antibody
Antibody SKU:	CAB7221
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 656-800 of human TAF5 (NP_008882.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 656-800 of human TAF5 (NP_008882.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VLNG NCVR IFTG HKGP IHSL TFSP NGRF LATG ATDG RVLL WDIG HGLM VGEL KGHT DTVC SLRF SRDG EILA SGSM DNTV RLWD AIKA FEDL ETDD FTTA TGHI NLPE NSQE LLLG TYMT KSTP VVHL HFTR RNLV LAAG AYSP Q
Gene ID:	6877
Uniprot:	Q15542
Cellular Location:	Nucleus
Calculated MW:	80kDa/86kDa
Observed MW:	100kDa

Synonyms:

TAF5, TAF(II)100, TAF2D, TAFII-100, TAFII100

Background:

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	TAF5: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. TAF5/TAFII100 interacts strongly with the histone H4-related TAF6/TAFII80 and the histone H3-related TAF9/TAFII31, as well as a stable complex comprised of both TAF5/TAFII80 and TAF6/TAFII31. Apparently weaker interactions of TAF5/TAFII100 with TBP, TAF1/TAFII250, TAF11/TAFII28, and TAF12/TAFII20, but not TAF7/TAFII55, also have been observed. Belongs to the WD repeat TAF5 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 10q24-q25.2 Cellular Component: nucleoplasm; transcription factor TFIID complex; intracellular membrane-bound organelle; nucleolus; nucleus; actin cytoskeleton Molecular Function:protein dimerization activity; protein binding; histone acetyltransferase activity; transcription factor activity Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; regulation of transcription, DNA-dependent; transcription initiation; RNA elongation from RNA polymerase II promoter; gene expression; chromatin modification; histone acetylation
NCBI Summary:	Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes an integral subunit of TFIID associated with all transcriptionally competent forms of that complex. This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15542
NCBI GenInfo Identifier:	78103206
NCBI Gene ID:	6877
NCBI Accession:	Q15542.3
UniProt Secondary Accession:	Q15542,Q53EM4, Q5SYD5, Q86UZ7, Q9Y4K5, A8K5B4, B2RMR0 B7ZKJ6,
UniProt Related Accession:	Q15542
Molecular Weight:	800
NCBI Full Name:	Transcription initiation factor TFIID subunit 5
NCBI Synonym Full Names:	TAF5 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 100kDa
NCBI Official Symbol:	TAF5
NCBI Official Synonym Symbols:	TAF2D; TAFII100
NCBI Protein Information:	transcription initiation factor TFIID subunit 5; TAFII-100; TAF(II)100; TATA box binding protein (TBP)-associated factor 2D; transcription initiation factor TFIID 100 kD subunit; transcription initiation factor TFIID 100 kDa subunit; TATA box binding protein (TBP)-associated factor, RNA polymerase II, D, 100kD
UniProt Protein Name:	Transcription initiation factor TFIID subunit 5
UniProt Synonym Protein Names:	Transcription initiation factor TFIID 100 kDa subunit; TAF(II)100; TAFII-100; TAFII100
Protein Family:	Transcription initiation factor
UniProt Gene Name:	TAF5
UniProt Entry Name:	TAF5_HUMAN

	Western blot analysis of extracts of mouse brain, using TAF5 antibody (CAB7221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A-549 cells using TAF5 antibody (CAB7221). Blue: DAPI for nuclear staining.