Antibody Name:	Anti-TAF1C Antibody
Antibody SKU:	CAB6759
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 70-320 of human TAF1C (NP_001230085.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, HT-1080, 293T, MCF7, Mouse thymus, Mouse eye, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 70-320 of human TAF1C (NP_001230085.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PPLI DPWD PGLT ARDL LFRG GYRY RKRP RVVL DVTE QISR FLLD HGDV AFAP LGKL MLEN FKLE GAGS RTKK KTVV SVKK LLQD LGGH QPWG CPWA YLSN RQRR FSIL GGPI LGTS VASH LAEL LHEE LVLR WEQL LLDE ACTG GALA WVPG RTPQ FGQL VYPA GGAQ DRLH FQEV VLTP GDNP QFLG KPGR IQLQ GPVR QVVT CTVQ GETL LAVR SDYH CAVW KFGK QWQP TLLQ AMQV EKGA TGIS LSP
Gene ID:	9013
Uniprot:	Q15572
Cellular Location:	Nucleus
Calculated MW:	49kDa/58kDa/84kDa/92kDa/95kDa
Observed MW:	110kDa

Synonyms:

TAF1C, MGC:39976, SL1, TAFI110, TAFI95

Background:

Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the largest SL1-specific TAF. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

UniProt Protein Function:	TAF1C: Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits. Recruits RNA polymerase I to the rRNA gene promoter via interaction with RRN3. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription initiation complex Chromosomal Location of Human Ortholog: 16q24 Cellular Component: nucleoplasm Molecular Function:protein binding; DNA binding Biological Process: chromatin silencing at rDNA; transcription from RNA polymerase II promoter; negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; termination of RNA polymerase I transcription; transcription initiation from RNA polymerase I promoter; regulation of gene expression, epigenetic
NCBI Summary:	Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the largest SL1-specific TAF. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2011]
UniProt Code:	Q15572
NCBI GenInfo Identifier:	313104019
NCBI Gene ID:	9013
NCBI Accession:	Q15572.2
UniProt Secondary Accession:	Q15572,Q59F67, Q8N6V3, B7Z5K5, B7Z5S4, B7Z908, B7Z9L7
UniProt Related Accession:	Q15572
Molecular Weight:	869
NCBI Full Name:	TATA box-binding protein-associated factor RNA polymerase I subunit C
NCBI Synonym Full Names:	TATA box binding protein (TBP)-associated factor, RNA polymerase I, C, 110kDa
NCBI Official Symbol:	TAF1C
NCBI Official Synonym Symbols:	SL1; TAFI95; TAFI110; MGC:39976
NCBI Protein Information:	TATA box-binding protein-associated factor RNA polymerase I subunit C; SL1, 110kD subunit; TBP-associated factor 1C; transcription factor SL1; TATA box-binding protein-associated factor 1C; transcription initiation factor SL1/TIF-IB subunit C; RNA polymerase I-specific TBP-associated factor 110 kDa; TATA box binding protein (TBP)-associated factor, RNA polymerase I, C, 110kD
UniProt Protein Name:	TATA box-binding protein-associated factor RNA polymerase I subunit C
UniProt Synonym Protein Names:	RNA polymerase I-specific TBP-associated factor 110 kDa; TAFI110; TATA box-binding protein-associated factor 1C; TBP-associated factor 1C; Transcription initiation factor SL1/TIF-IB subunit C
Protein Family:	TATA box-binding protein-associated factor
UniProt Gene Name:	TAF1C
UniProt Entry Name:	TAF1C_HUMAN

	Western blot analysis of extracts of various cell lines, using TAF1C antibody (CAB6759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse lung using TAF1C Antibody (CAB6759) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using TAF1C Antibody (CAB6759) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using TAF1C Antibody (CAB6759) at dilution of 1:100 (40x lens).