Antibody Name:	Anti-TAF11 Antibody
Antibody SKU:	CAB7934
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human TAF11 (NP_005634.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, SW620

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human TAF11 (NP_005634.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDDA HESP SDKG GETG ESDE TAAV PGDP GATD TDGI PEET DGDA DVDL KEAA AEEG ELES QDVS DLTT VERE DSSL LNPA AKKL KIDT KEKK EKKQ KVDE DEIQ KMQI LVSS FSEE QLNR YEMY RRSA FPKA AIKR LIQS ITGT SVSQ NVVI AMSG ISKV FVGE VVEE ALDV CEKW GEMP PLQP KHMR EAVR RLKS KGQI PNSK HKKI IFF
Gene ID:	6882
Uniprot:	Q15544
Cellular Location:	Nucleus
Calculated MW:	17kDa/23kDa
Observed MW:	23kDa

Synonyms:

TAF11, MGC:15243, PRO2134, TAF2I, TAFII28

Background:

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a small subunit of TFIID that is present in all TFIID complexes and interacts with TBP. This subunit also interacts with another small subunit, TAF13, to form a heterodimer with a structure similar to the histone core structure. Two transcript variants encoding different isoforms have been found for this gene.

NCBI Summary:	Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a small subunit of TFIID that is present in all TFIID complexes and interacts with TBP. This subunit also interacts with another small subunit, TAF13, to form a heterodimer with a structure similar to the histone core structure. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:	Q15544
NCBI GenInfo Identifier:	5032151
NCBI Gene ID:	6882
NCBI Accession:	NP_005634
UniProt Related Accession:	Q15544
Molecular Weight:	23kDa
NCBI Full Name:	transcription initiation factor TFIID subunit 11 isoform 1
NCBI Synonym Full Names:	TATA-box binding protein associated factor 11
NCBI Official Symbol:	TAF11
NCBI Official Synonym Symbols:	TAF2I; PRO2134; TAFII28; MGC:15243
NCBI Protein Information:	transcription initiation factor TFIID subunit 11
UniProt Protein Name:	Transcription initiation factor TFIID subunit 11
UniProt Synonym Protein Names:	TFIID subunit p30-beta; Transcription initiation factor TFIID 28 kDa subunit
Protein Family:	Transcription initiation factor
UniProt Gene Name:	TAF11
UniProt Entry Name:	TAF11_HUMAN

	Western blot analysis of extracts of various cell lines, using TAF11 antibody (CAB7934) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using TAF11 antibody (CAB7934) at dilution of 1:100. Blue: DAPI for nuclear staining.