Cell Cycle Antibodies 1

Anti-TAF1 Antibody (CAB15730)

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SKU:
CAB15730
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-TAF1 Antibody
Antibody SKU:CAB15730
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1630-1893 of human TAF1 (NP_004597.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, Jurakt, HeLa, NIH/3T3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1630-1893 of human TAF1 (NP_004597.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EHLT QLEK DICT AKEA ALEE AELE SLDP MTPG PYTP QPPD LYDT NTSL SMSR DASV FQDE SNMS VLDI PSAT PEKQ VTQE GEDG DGDL ADEE EGTV QQPQ ASVL YEDL LMSE GEDD EEDA GSDE EGDN PFSA IQLS ESGS DSDV GSGG IRPK QPRM LQEN TRMD MENE ESMM SYEG DGGE ASHG LEDS NISY GSYE EPDP KSNT QDTS FSSI GGYE VSEE EEDE EEEE QRSG PSVL SQVH LSED EEDS EDFH SIAG DSDL DSDE
Gene ID:6872
Uniprot:P21675
Cellular Location:Nucleus
Calculated MW:174-182kDa, 204-216kDa
Observed MW:300kDa
Synonyms:TAF1, BA2R, CCG1, CCGS, DYT3, DYT3/TAF1, KAT4, MRXS33, N-TAF1, NSCL2, OF, P250, TAF(II)250, TAF2A, TAFII-250, TAFII250, XDP
Background:Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons.
UniProt Protein Function:TAF1: Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA- binding activity. Essential for progression of the G1 phase of the cell cycle. Defects in TAF1 are the cause of dystonia type 3 (DYT3); also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease. Belongs to the TAF1 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Kinase, protein; Protein kinase, atypical; EC 2.7.11.1; DNA-binding; Transcription, coactivator/corepressor; EC 2.3.1.48; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; TAF1 family

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: nucleoplasm; transcription factor TFIID complex

Molecular Function:histone acetyltransferase activity; p53 binding; protein binding; protein serine/threonine kinase activity; sequence-specific DNA binding; TATA-binding protein binding; transcription coactivator activity; transcription factor binding

Biological Process: peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; protein amino acid autophosphorylation; response to DNA damage stimulus; RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation; transcription initiation from RNA polymerase II promoter; transcriptional preinitiation complex assembly

Disease: Dystonia 3, Torsion, X-linked

NCBI Summary:Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes the largest subunit of TFIID. This subunit binds to core promoter sequences encompassing the transcription start site. It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. This subunit contains two independent protein kinase domains at the N- and C-terminals, but also possesses acetyltransferase activity and can act as a ubiquitin-activating/conjugating enzyme. Mutations in this gene result in Dystonia 3, torsion, X-linked, a dystonia-parkinsonism disorder. Alternative splicing of this gene results in multiple transcript variants. This gene is part of a complex transcription unit (TAF1/DYT3), wherein some transcript variants share exons with TAF1 as well as additional downstream DYT3 exons. [provided by RefSeq, Oct 2013]
UniProt Code:P21675
NCBI GenInfo Identifier:115942
NCBI Gene ID:6872
NCBI Accession:P21675.2
UniProt Secondary Accession:P21675,Q59FZ3, Q6IUZ1, Q70Q86, Q70Q87, Q70T00, Q70T01 A5CVC8, A5CVC9, A5CVD0, A5CVD1, B1Q2X3,
UniProt Related Accession:P21675
Molecular Weight:214,913 Da
NCBI Full Name:Transcription initiation factor TFIID subunit 1
NCBI Synonym Full Names:TATA-box binding protein associated factor 1
NCBI Official Symbol:TAF1  
NCBI Official Synonym Symbols:OF; XDP; BA2R; CCG1; CCGS; DYT3; KAT4; P250; NSCL2; TAF2A; MRXS33; N-TAF1; TAFII250; DYT3/TAF1; TAFII-250; TAF(II)250  
NCBI Protein Information:transcription initiation factor TFIID subunit 1
UniProt Protein Name:Transcription initiation factor TFIID subunit 1
UniProt Synonym Protein Names:Cell cycle gene 1 protein; TBP-associated factor 250 kDa; p250; Transcription initiation factor TFIID 250 kDa subunit; TAF(II)250; TAFII-250; TAFII250
Protein Family:Taz1-interacting factor
UniProt Gene Name:TAF1  
UniProt Entry Name:TAF1_HUMAN
Anti-TAF1 Antibody (CAB15730)Western blot analysis of extracts of various cell lines, using TAF1 antibody (CAB15730) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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