Cell Biology Antibodies 9
Anti-TAB1 Antibody (CAB5749)
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- SKU:
- CAB5749
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-TAB1 Antibody |
| Antibody SKU: | CAB5749 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF IP |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 205-504 of human TAB1 (NP_006107.1). |
| Application: | WB IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HL-60, MCF7, A-431, U-251MG, HeLa, Mouse kidney, Mouse heart, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 205-504 of human TAB1 (NP_006107.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VDHT TENE DELF RLSQ LGLD AGKI KQVG IICG QEST RRIG DYKV KYGY TDID LLSA AKSK PIIA EPEI HGAQ PLDG VTGF LVLM SEGL YKAL EAAH GPGQ ANQE IAAM IDTE FAKQ TSLD AVAQ AVVD RVKR IHSD TFAS GGER ARFC PRHE DMTL LVRN FGYP LGEM SQPT PSPA PAAG GRVY PVSV PYSS AQST SKTS VTLS LVMP SQGQ MVNG AHSA STLD EATP TLTN QSPT LTLQ STNT HTQS SSSS SDGG LFRS RPAH SLPP GEDG RVEP YVDF AEFY RLWS VDHG EQSV VTAP |
| Gene ID: | 10454 |
| Uniprot: | Q15750 |
| Cellular Location: | |
| Calculated MW: | 49kDa/54kDa |
| Observed MW: | 70kDa |
| Synonyms: | TAB1, 3'-Tab1, MAP3K7IP1 |
| Background: | The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. Alternatively spliced transcript variants encoding distinct isoforms have been reported. |
| UniProt Protein Function: | TAB1: May be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis. Interacts with XIAP and BIRC7. Interacts with TRAF6 and MAP3K7; during IL-1 signaling. Identified in the TRIKA2 complex composed of MAP3K7, TAB1 and TAB2. Ubiquitous. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Activator; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: nucleoplasm; protein complex; cytoplasm; endosome membrane; cytosol Molecular Function:protein binding; mitogen-activated protein kinase p38 binding; enzyme activator activity; protein complex binding; kinase activator activity; catalytic activity Biological Process: heart morphogenesis; I-kappaB kinase/NF-kappaB cascade; activation of MAPK activity; in utero embryonic development; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; transforming growth factor beta receptor signaling pathway; toll-like receptor signaling pathway; activation of MAPKKK activity; JNK cascade; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway; lung development |
| NCBI Summary: | The protein encoded by this gene was identified as a regulator of the MAP kinase kinase kinase MAP3K7/TAK1, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta, interleukin 1, and WNT-1. This protein interacts and thus activates TAK1 kinase. It has been shown that the C-terminal portion of this protein is sufficient for binding and activation of TAK1, while a portion of the N-terminus acts as a dominant-negative inhibitor of TGF beta, suggesting that this protein may function as a mediator between TGF beta receptors and TAK1. This protein can also interact with and activate the mitogen-activated protein kinase 14 (MAPK14/p38alpha), and thus represents an alternative activation pathway, in addition to the MAPKK pathways, which contributes to the biological responses of MAPK14 to various stimuli. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15750 |
| NCBI GenInfo Identifier: | 10720303 |
| NCBI Gene ID: | 10454 |
| NCBI Accession: | Q15750.1 |
| UniProt Secondary Accession: | Q15750,Q2PP09, Q8IZW2, |
| UniProt Related Accession: | Q15750 |
| Molecular Weight: | 504 |
| NCBI Full Name: | TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 |
| NCBI Synonym Full Names: | TGF-beta activated kinase 1/MAP3K7 binding protein 1 |
| NCBI Official Symbol: | TAB1Â Â |
| NCBI Official Synonym Symbols: | 3'-Tab1; MAP3K7IP1Â Â |
| NCBI Protein Information: | TGF-beta-activated kinase 1 and MAP3K7-binding protein 1; TAK1-binding protein 1; transforming growth factor beta-activated kinase-binding protein 1; mitogen-activated protein kinase kinase kinase 7-interacting protein 1 |
| UniProt Protein Name: | TGF-beta-activated kinase 1 and MAP3K7-binding protein 1 |
| UniProt Synonym Protein Names: | Mitogen-activated protein kinase kinase kinase 7-interacting protein 1; TGF-beta-activated kinase 1-binding protein 1; TAK1-binding protein 1 |
| Protein Family: | TGF-beta-activated kinase 1 and MAP3K7-binding protein |
| UniProt Gene Name: | TAB1Â Â |
| UniProt Entry Name: | TAB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using TAB1 antibody (CAB5749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using TAB1 antibody (CAB5749). Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug TAB1 antibody (CAB5749). Western blot was performed from the immunoprecipitate using TAB1 antibody (CAB5749) at a dilition of 1:1000. |
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