Epigenetics & Nuclear Signaling Antibodies 3

Anti-T Antibody (CAB16887)

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SKU:
CAB16887
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-T Antibody
Antibody SKU:CAB16887
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 226-435 of human T (NP_003172.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human
Positive Samples:H460
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 226-435 of human T (NP_003172.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HKEM MEEP GDSQ QPGY SQWG WLLP GTST LCPP ANPH PQFG GALS LPST HSCD RYPT LRSH RSSP YPSP YAHR NNSP TYSD NSPA CLSM LQSH DNWS SLGM PAHP SMLP VSHN ASPP TSSS QYPS LWSV SNGA VTPG SQAA AVSN GLGA QFFR GSPA HYTP LTHP VSAP SSSG SPLY EGAA AATD IVDS QYDA AAQG RLIA SWTP VSPP SM
Gene ID:6862
Uniprot:O15178
Cellular Location:Nucleus
Calculated MW:41kDa/47kDa
Observed MW:47kDa
Synonyms:T, SAVA, TFT
Background:The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:Brachyury: Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site. Genetic variations in T are associated with susceptibility to neural tube defects (NTD). NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis. T is involved in susceptibility to the development of chordoma (CHDM). Chordomas are rare, clinically malignant tumors derived from notochordal remnants. They occur along the length of the spinal axis, predominantly in the sphenooccipital, vertebral and sacrococcygeal regions. They are characterized by slow growth, local destruction of bone, extension into adjacent soft tissues and rarely, distant metastatic spread. Susceptibility to development of chordomas is due to a T gene duplication.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; DNA-binding; Cell cycle regulation; Transcription factor

Chromosomal Location of Human Ortholog: 6q27

Cellular Component: cytoplasm; nuclear chromatin; nucleus

Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; heart morphogenesis; somitogenesis; neural plate morphogenesis; embryonic skeletal development; mesoderm migration; notochord formation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; signal transduction; BMP signaling pathway; determination of anterior/posterior axis, embryo; positive regulation of cell proliferation; neural tube closure; penetration of zona pellucida; mesoderm development; vasculogenesis

Disease: Neural Tube Defects; Sacral Agenesis With Vertebral Anomalies

NCBI Summary:The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:O15178
NCBI GenInfo Identifier:4507339
NCBI Gene ID:6862
NCBI Accession:NP_003172.1
UniProt Secondary Accession:O15178,Q4KMP4, E7ERD6,
UniProt Related Accession:O15178
Molecular Weight:41,078 Da
NCBI Full Name:brachyury protein isoform 1
NCBI Synonym Full Names:T brachyury transcription factor
NCBI Official Symbol:T  
NCBI Official Synonym Symbols:TFT; SAVA  
NCBI Protein Information:brachyury protein
UniProt Protein Name:Brachyury protein
UniProt Synonym Protein Names:Protein T
Protein Family:THP3
UniProt Gene Name:T  
UniProt Entry Name:BRAC_HUMAN
Anti-T Antibody (CAB16887)Western blot analysis of extracts of H460 cells, using T antibody (CAB16887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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