Name: Anti-SYVN1 Antibody (CAB2605)
Brand: Metabolism Antibodies 2
SKU: CAB2605
Availability: OutOfStock

Antibody Name:	Anti-SYVN1 Antibody
Antibody SKU:	CAB2605
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human SYVN1 (NP_757385.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HeLa, HepG2, Mouse pancreas, Mouse lung, Mouse kidney

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human SYVN1 (NP_757385.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LEAR LQSL RNIH TLLD AAML QINQ YLTV LASL GPPR PATS VNST EETA TTVV AAAS STSI PSSE ATTP TPGA SPPA PEME RPPA PESV GTEE MPED GEPD AAEL RRRR LQKL ESPV AH
Gene ID:	84447
Uniprot:	Q86TM6
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	61kDa/67kDa
Observed MW:	70kDa

Synonyms:

SYVN1, DER3, HRD1

Background:

This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms.

UniProt Protein Function:	SYVN1: Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. Belongs to the HRD1 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system; EC 6.3.2.-; Endoplasmic reticulum; Ligase; Ubiquitin ligase; Membrane protein, multi-pass; EC 6.3.2.19; Membrane protein, integral Chromosomal Location of Human Ortholog: 11q13 Cellular Component: nucleoplasm; endoplasmic reticulum membrane; membrane; endoplasmic reticulum; integral to membrane Molecular Function:protein binding; acid-amino acid ligase activity; zinc ion binding Biological Process: ER-associated protein catabolic process; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; unfolded protein response; in utero embryonic development; protein ubiquitination; protein amino acid N-linked glycosylation via asparagine
NCBI Summary:	This gene encodes a protein involved in endoplasmic reticulum (ER)-associated degradation. The encoded protein removes unfolded proteins, accumulated during ER stress, by retrograde transport to the cytosol from the ER. This protein also uses the ubiquitin-proteasome system for additional degradation of unfolded proteins. Sequence analysis identified two transcript variants that encode different isoforms. [provided by RefSeq, May 2011]
UniProt Code:	Q86TM6
NCBI GenInfo Identifier:	134035039
NCBI Gene ID:	84447
NCBI Accession:	Q86TM6.2
UniProt Secondary Accession:	Q86TM6,Q8N3K3, Q8N6E8, Q96JL5, Q96PK3,
UniProt Related Accession:	Q86TM6
Molecular Weight:	617
NCBI Full Name:	E3 ubiquitin-protein ligase synoviolin
NCBI Synonym Full Names:	synovial apoptosis inhibitor 1, synoviolin
NCBI Official Symbol:	SYVN1
NCBI Official Synonym Symbols:	DER3; HRD1
NCBI Protein Information:	E3 ubiquitin-protein ligase synoviolin; HMG-coA reductase degradation 1 homolog
UniProt Protein Name:	E3 ubiquitin-protein ligase synoviolin
UniProt Synonym Protein Names:	Synovial apoptosis inhibitor 1
Protein Family:	E3 ubiquitin-protein ligase
UniProt Gene Name:	SYVN1
UniProt Entry Name:	SYVN1_HUMAN

Western blot analysis of extracts of various cell lines, using SYVN1 antibody (CAB2605) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

SYVN1 Antibody (PACO12616)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)