Cell Biology Antibodies 5

Anti-SYT5 Antibody (CAB15321)

(No reviews yet) Write a Review
SKU:
CAB15321
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-SYT5 Antibody
Antibody SKU:CAB15321
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human SYT5 (NP_003171.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human SYT5 (NP_003171.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MFPE PPTP GPPS PDTP PDSS RISH GPVP PWAL ATIV LVSG LLIF SCCF CLYR KSCR RRTG KKSQ AQAQ VHLQ EVKG LGQS YIDK VQPE VEEL EPAP SGPG QQVA DKHE LG
Gene ID:6861
Uniprot:O00445
Cellular Location:Cytoplasmic vesicle, Recycling endosome membrane, Single-pass membrane protein, secretory vesicle, synaptic vesicle membrane
Calculated MW:42kDa
Observed MW:43kDa
Synonyms:SYT5
Background:
UniProt Protein Function:SYT5: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)- dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface. Belongs to the synaptotagmin family.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.42|11p

Cellular Component: plasma membrane; synaptic vesicle membrane

Molecular Function:calcium ion binding; calcium-dependent phospholipid binding; clathrin binding; syntaxin binding

Biological Process: calcium ion-dependent exocytosis of neurotransmitter; regulation of calcium ion-dependent exocytosis; synaptic transmission; synaptic vesicle endocytosis; vesicle fusion

NCBI Summary:Synaptotagmins, such as SYT5, are a family of type III membrane proteins characterized by cytoplasmic repeats related to protein kinase C (see MIM 176960) regulatory (C2) domains, which are thought to bind calcium. Synaptotagmins may act both as negative regulators of vesicle fusion, allowing fusion in the presence of calcium, and as calcium receptors or sensor molecules (summary by Hudson and Birnbaum, 1995 [PubMed 7597049]).[supplied by OMIM, Feb 2011]
UniProt Code:O00445
NCBI GenInfo Identifier:32700066
NCBI Gene ID:6861
NCBI Accession:O00445.2
UniProt Secondary Accession:O00445,Q86X72, B3KWJ8, B7Z300,
UniProt Related Accession:O00445
Molecular Weight:42,474 Da
NCBI Full Name:Synaptotagmin-5
NCBI Synonym Full Names:synaptotagmin 5
NCBI Official Symbol:SYT5  
NCBI Protein Information:synaptotagmin-5
UniProt Protein Name:Synaptotagmin-5
UniProt Synonym Protein Names:Synaptotagmin V; SytV
Protein Family:Synaptotagmin
UniProt Gene Name:SYT5  
UniProt Entry Name:SYT5_HUMAN
Anti-SYT5 Antibody (CAB15321)Western blot analysis of extracts of Mouse kidney, using SYT5 antibody (CAB15321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
View AllClose

0 Reviews

View AllClose

Related Products