Name: Anti-SYT1 Antibody (CAB0992)
Brand: Developmental Biology
SKU: CAB0992
Availability: OutOfStock

Antibody Name:	Anti-SYT1 Antibody
Antibody SKU:	CAB0992
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-422 of human SYT1 (NP_001129278.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, A673

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-422 of human SYT1 (NP_001129278.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVSE SHHE ALAA PPVT TVAT VLPS NATE PASP GEGK EDAF SKLK EKFM NELH KIPL PPWA LIAI AIVA VLLV LTCC FCIC KKCL FKKK NKKK GKEK GGKN AINM KDVK DLGK TMKD QALK DDDA ETGL TDGE EKEE PKEE EKLG KLQY SLDY DFQN NQLL VGII QAAE LPAL DMGG TSDP YVKV FLLP DKKK KFET KVHR KTLN PVFN EQFT FKVP YSEL GGKT LVMA VYDF DRFS KHDI IGEF KVPM NTVD FGHV TEEW RDLQ SAEK EEQE KLGD ICFS LRYV PTAG KLTV VILE AKNL KKMD VGGL SDPY VKIH LMQN GKRL KKKK TTIK KNTL NPYY NESF SFEV PFEQ IQKV QVVV TVLD YDKI GKND AIGK VFVG YNST GAEL RHWS DMLA NPRR PIAQ WHTL QVEE EVDA MLAV KK
Gene ID:	6857
Uniprot:	P21579
Cellular Location:	Cytoplasm, Cytoplasmic vesicle, Single-pass membrane protein, chromaffin granule membrane, secretory vesicle, synaptic vesicle membrane
Calculated MW:	47kDa
Observed MW:	60kDa

Synonyms:	SYT1, P65, SVP65, SYT
Background:

UniProt Protein Function:	SYT1: an integral membrane protein of synaptic vesicles thought to serve as a Ca(2+) sensor in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse. Binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
UniProt Protein Details:	Protein type:Membrane protein, integral; Vesicle; Calcium-binding Chromosomal Location of Human Ortholog: 12cen-q21 Cellular Component: neuron projection; plasma membrane; synaptic vesicle; synaptic vesicle membrane Molecular Function:calcium ion binding; calcium-dependent phospholipid binding; clathrin binding; low-density lipoprotein receptor binding; phosphatidylinositol binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding; SNARE binding; syntaxin-1 binding Biological Process: calcium ion-dependent exocytosis of neurotransmitter; detection of calcium ion; glutamate secretion; neurotransmitter secretion; positive regulation of synaptic transmission; protein homooligomerization; regulation of calcium ion-dependent exocytosis; regulation of exocytosis; regulation of synaptic transmission, glutamatergic; synaptic transmission; synaptic vesicle endocytosis; vesicle fusion
NCBI Summary:	The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]
UniProt Code:	P21579
NCBI GenInfo Identifier:	135086
NCBI Gene ID:	6857
NCBI Accession:	P21579.1
UniProt Secondary Accession:	P21579,Q6AI31,
UniProt Related Accession:	P21579
Molecular Weight:	47,573 Da
NCBI Full Name:	Synaptotagmin-1
NCBI Synonym Full Names:	synaptotagmin 1
NCBI Official Symbol:	SYT1
NCBI Official Synonym Symbols:	P65; SYT; SVP65
NCBI Protein Information:	synaptotagmin-1
UniProt Protein Name:	Synaptotagmin-1
UniProt Synonym Protein Names:	Synaptotagmin I; SytI; p65
Protein Family:	Synaptotagmin
UniProt Gene Name:	SYT1
UniProt Entry Name:	SYT1_HUMAN

	Western blot analysis of extracts of various cell lines, using SYT1 antibody (CAB0992) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
	Immunohistochemistry of paraffin-embedded rat kidney using SYT1 antibody (CAB0992) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of rat eye using SYT1 Rabbit pAb (CAB0992) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse eye using SYT1 Rabbit pAb (CAB0992) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.