Cell Biology Antibodies 12

Anti-Symplekin Antibody (CAB8722)

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SKU:
CAB8722
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-Symplekin Antibody
Antibody SKU:CAB8722
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 310-530 of human SYMPK (NP_004810.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse
Positive Samples:LO2, 293T, HL-60, HT-1080, A-549, Mouse brain, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 310-530 of human SYMPK (NP_004810.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:KHPA SLEF QAQI TTLL VDLG TPQA EIAR NMPS SKDT RKRP RDDS DSTL KKMK LEPN LGED DEDK DLEP GPSG TSKA SAQI SGQS DTDI TAEF LQPL LTPD NVAN LVLI SMVY LPEA MPAS FQAI YTPV ESAG TEAQ IKHL ARLM ATQM TAAG LGPG VEQT KQCK EEPK EEKV VKTE SVLI KRRL SAQG QAIS VVGS LSSM SPLE EEAP QAKR RPEP IIPV T
Gene ID:8189
Uniprot:Q92797
Cellular Location:Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Nucleus, Peripheral membrane protein, cytoskeleton, nucleoplasm, tight junction
Calculated MW:74kDa/141kDa
Observed MW:141kDa
Synonyms:SYMPK, SPK, SYM, symplekin
Background:This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.
NCBI Summary:This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types. [provided by RefSeq, Jul 2008]
UniProt Code:Q92797
NCBI GenInfo Identifier:124028529
NCBI Gene ID:8189
NCBI Accession:NP_004810.2
UniProt Related Accession:Q92797
Molecular Weight:141kDa
NCBI Full Name:symplekin
NCBI Synonym Full Names:symplekin
NCBI Official Symbol:SYMPK  
NCBI Official Synonym Symbols:SPK; SYM  
NCBI Protein Information:symplekin
UniProt Protein Name:Symplekin
Protein Family:Symplekin
UniProt Gene Name:SYMPK  
UniProt Entry Name:SYMPK_HUMAN
Anti-Symplekin Polyclonal Antibody (CAB8722)Western blot analysis of extracts of various cell lines, using SYMPK antibody (CAB8722) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
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