Immunology Antibodies 2
Anti-Syk Antibody (CAB2123)
- SKU:
- CAB2123
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-Syk Antibody |
| Antibody SKU: | CAB2123 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human Syk |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, Mouse spleen, Rat liver |
| Immunogen: | Recombinant protein of human Syk |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 6850 |
| Uniprot: | P43405 |
| Cellular Location: | Cell membrane, Cytoplasm, cytosol |
| Calculated MW: | 69kDa/72kDa |
| Observed MW: | 69kDa/72kDa |
| Synonyms: | SYK, p72-Syk |
| Background: | This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described. |
| UniProt Protein Details: | Protein type:EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; Protein kinase, TK; TK group; Syk family Chromosomal Location of Human Ortholog: 9q22 Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; protein complex; cytoplasm; plasma membrane; nucleus; cytosol; B cell receptor complex Molecular Function:integrin binding; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein kinase binding; ATP binding; protein kinase activity Biological Process: viral reproduction; transcription factor import into nucleus; positive regulation of interleukin-3 biosynthetic process; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of calcium-mediated signaling; regulation of phagocytosis; protein amino acid phosphorylation; activation of JNK activity; leukocyte adhesion; B cell receptor signaling pathway; regulation of transcription factor activity; positive regulation of bone resorption; beta selection; positive regulation of gamma-delta T cell differentiation; angiogenesis; inflammatory response; integrin-mediated signaling pathway; platelet activation; neutrophil chemotaxis; adaptive immune response; regulation of superoxide release; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; regulation of neutrophil degranulation; leukotriene biosynthetic process; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; organ morphogenesis; neutrophil activation during immune response; serotonin secretion by platelet; leukocyte activation during immune response; defense response to bacterium; macrophage activation during immune response; positive regulation of B cell differentiation; blood vessel morphogenesis; innate immune response; positive regulation of alpha-beta T cell proliferation; lymph vessel development; blood coagulation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of alpha-beta T cell differentiation; positive regulation of cytokine secretion |
| NCBI Summary: | This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010] |
| UniProt Code: | P43405 |
| NCBI GenInfo Identifier: | 1174527 |
| NCBI Gene ID: | 6850 |
| NCBI Accession: | P43405.1 |
| UniProt Related Accession: | P43405 |
| Molecular Weight: | 635 |
| NCBI Full Name: | Tyrosine-protein kinase SYK |
| NCBI Synonym Full Names: | spleen tyrosine kinase |
| NCBI Official Symbol: | SYKÂ Â |
| NCBI Official Synonym Symbols: | p72-Syk  |
| NCBI Protein Information: | tyrosine-protein kinase SYK |
| UniProt Protein Name: | Tyrosine-protein kinase SYK |
| UniProt Synonym Protein Names: | Spleen tyrosine kinase; p72-Syk |
| Protein Family: | Tyrosine-protein kinase |
| UniProt Gene Name: | SYKÂ Â |
| UniProt Entry Name: | KSYK_HUMAN |
 | Western blot analysis of extracts of Rat liver, using Syk antibody (CAB2123) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Western blot analysis of extracts of various cell lines, using Syk antibody (CAB2123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using Syk Antibody (CAB2123) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using Syk Antibody (CAB2123) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using Syk Antibody (CAB2123) at dilution of 1:100 (40x lens). |
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