Immunology Antibodies 1
Anti-SWAP70 Antibody (CAB14857)
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- SKU:
- CAB14857
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-SWAP70 Antibody |
| Antibody SKU: | CAB14857 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 316-585 of human SWAP70 (NP_055870.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, A-549, LO2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 316-585 of human SWAP70 (NP_055870.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | HKEA RQRR KELR KKQL AEQE ELER QMKE LQAA NESK QQEL EAVR KKLE EAAS RAAE EEKK RLQT QVEL QARF STEL EREK LIRQ QMEE QVAQ KSSE LEQY LQRV RELE DMYL KLQE ALED ERQA RQDE ETVR KLQA RLLE EESS KRAE LEKW HLEQ QQAI QTTE AEKQ ELEN QRVL KEQA LQEA MEQL EQLE LERK QALE QYEE VKKK LEMA TNKT KSWK DKVA HHEG LIRL IEPG SKNP HLIT NWGP AAFT EAEL EERE KNWK EKKT TE |
| Gene ID: | 23075 |
| Uniprot: | Q9UH65 |
| Cellular Location: | Cell membrane, Cell projection, Cytoplasm, Nucleus, lamellipodium |
| Calculated MW: | 68kDa |
| Observed MW: | 69kDa |
| Synonyms: | SWAP70, HSPC321, SWAP-70 |
| Background: |
| UniProt Protein Function: | SWAP-70: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion. |
| UniProt Protein Details: | Protein type:GEF; GEF, Rac/Rho Chromosomal Location of Human Ortholog: 11p15.4 Cellular Component: cell-cell adherens junction; cytoplasm Molecular Function:protein binding |
| UniProt Code: | Q9UH65 |
| NCBI GenInfo Identifier: | 74753323 |
| NCBI Gene ID: | 23075 |
| NCBI Accession: | Q9UH65.1 |
| UniProt Secondary Accession: | Q9UH65,O75135, Q7LCY6, Q9P061, Q9P0Z8, D3DQV1, |
| UniProt Related Accession: | Q9UH65 |
| Molecular Weight: | 68,998 Da |
| NCBI Full Name: | Switch-associated protein 70 |
| NCBI Synonym Full Names: | SWAP switching B-cell complex subunit 70 |
| NCBI Official Symbol: | SWAP70 |
| NCBI Official Synonym Symbols: | HSPC321; SWAP-70 |
| NCBI Protein Information: | switch-associated protein 70 |
| UniProt Protein Name: | Switch-associated protein 70 |
| Protein Family: | Switch-associated protein |
| UniProt Gene Name: | SWAP70 |
 | Western blot analysis of extracts of various cell lines, using SWAP70 antibody (CAB14857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded Mouse spleen using SWAP70 Rabbit pAb (CAB14857) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Rat spleen using SWAP70 Rabbit pAb (CAB14857) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded Human lung using SWAP70 Rabbit pAb (CAB14857) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of L929 cells using SWAP70 antibody (CAB14857) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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