Cardiovascular Antibodies

Anti-SUV420H1 Antibody (CAB15442)

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SKU:
CAB15442
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cardiovascular

Description

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Antibody Name:Anti-SUV420H1 Antibody
Antibody SKU:CAB15442
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human SUV420H1 (NP_060105.3).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, HeLa, SW480, Mouse uterus, Mouse lung, Rat uterus
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human SUV420H1 (NP_060105.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MNTS AFPS RSSR HFSK SDSF SHNN PVRF RPIK GRQE ELKE VIER FKKD EHLE KAFK CLTS GEWA RHYF LNKN KMQE KLFK EHVF IYLR MFAT DSGF EILP C
Gene ID:51111
Uniprot:Q4FZB7
Cellular Location:Chromosome, Nucleus
Calculated MW:31kDa/44kDa/99kDa
Observed MW:110kDa
Synonyms:KMT5B, CGI-85, CGI85, SUV420H1
Background:This gene encodes a protein that contains a SET domain. SET domains appear to be protein-protein interaction domains that mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). The function of this gene has not been determined. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:SUV420H1: a protein methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Strongly down-regulated in breast cancer cells. Belongs to the histone-lysine methyltransferase family. Suvar4-20 subfamily. Three alternatively-spliced isoforms have been described.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: nucleoplasm; nucleus

Molecular Function:histone-lysine N-methyltransferase activity

NCBI Summary:This gene encodes a protein that contains a SET domain. SET domains appear to be protein-protein interaction domains that mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). The function of this gene has not been determined. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
UniProt Code:Q4FZB7
NCBI GenInfo Identifier:332278247
NCBI Gene ID:51111
NCBI Accession:Q4FZB7.4
UniProt Secondary Accession:Q4FZB7,Q3SX56, Q4V775, Q6P150, Q96E44, Q9BUL0, Q9H022 Q9H2K3, Q9NXV3, Q9Y393, B7WNX7,
UniProt Related Accession:Q4FZB7
Molecular Weight:Calculated: 31kDa/44kDa/99kDaObserved: 92kDa
NCBI Full Name:Histone-lysine N-methyltransferase KMT5B
NCBI Synonym Full Names:lysine methyltransferase 5B
NCBI Official Symbol:KMT5B  
NCBI Official Synonym Symbols:CGI85; CGI-85; SUV420H1  
NCBI Protein Information:histone-lysine N-methyltransferase KMT5B
UniProt Protein Name:Histone-lysine N-methyltransferase KMT5B
UniProt Synonym Protein Names:Lysine N-methyltransferase 5B; Lysine-specific methyltransferase 5B
Protein Family:Histone-lysine N-methyltransferase
UniProt Gene Name:KMT5B  
Anti-SUV420H1 Antibody (CAB15442)Western blot analysis of extracts of various cell lines, using SUV420H1 antibody (CAB15442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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