Name: Anti-SUV39H2 Antibody (CAB5855)
Brand: Cell Biology Antibodies 9
SKU: CAB5855
Availability: OutOfStock

Antibody Name:	Anti-SUV39H2 Antibody
Antibody SKU:	CAB5855
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP ChIP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 141-350 of human SUV39H2 (NP_001180354.1).

Application:	WB IF IP ChIP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200 ChIP 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, MCF-7, U-937, 293T, HeLa, Mouse heart, Mouse liver, Mouse eye

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 141-350 of human SUV39H2 (NP_001180354.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CCPA EAGV LLAY NKNQ QIKI PPGT PIYE CNSR CQCG PDCP NRIV QKGT QYSL CIFR TSNG RGWG VKTL VKIK RMSF VMEY VGEV ITSE EAER RGQF YDNK GITY LFDL DYES DEFT VDAA RYGN VSHF VNHS CDPN LQVF NVFI DNLD TRLP RIAL FSTR TINA GEEL TFDY QMKG SGDI SSDS IDHS PAKK RVRT VCKC GAVT CRGY LN
Gene ID:	79723
Uniprot:	Q9H5I1
Cellular Location:	Chromosome, Nucleus, centromere
Calculated MW:	26kDa/39kDa/46kDa
Observed MW:	48kDa

Synonyms:	SUV39H2, KMT1B
Background:

UniProt Protein Function:	SUV39H2: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys- 9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.1.1.43; Methyltransferase; Methyltransferase, protein lysine; Amino Acid Metabolism - lysine degradation Chromosomal Location of Human Ortholog: 10p13 Cellular Component: nucleoplasm; nuclear heterochromatin; chromatin; chromosome, pericentric region Molecular Function:protein binding; zinc ion binding; histone lysine N-methyltransferase activity (H3-K9 specific); chromatin binding Biological Process: chromatin remodeling; chromatin assembly or disassembly; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; rhythmic process; negative regulation of circadian rhythm; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; male meiosis; cell differentiation
UniProt Code:	Q9H5I1
NCBI GenInfo Identifier:	25091325
NCBI Gene ID:	79723
NCBI Accession:	Q9H5I1.2
UniProt Secondary Accession:	Q9H5I1,Q5JSS4, Q5JSS5, Q6I9Y3, Q8ND06, D3DRT4,
UniProt Related Accession:	Q9H5I1
Molecular Weight:	410
NCBI Full Name:	Histone-lysine N-methyltransferase SUV39H2
NCBI Synonym Full Names:	suppressor of variegation 3-9 homolog 2 (Drosophila)
NCBI Official Symbol:	SUV39H2
NCBI Official Synonym Symbols:	KMT1B
NCBI Protein Information:	histone-lysine N-methyltransferase SUV39H2; H3-K9-HMTase 2; su(var)3-9 homolog 2; lysine N-methyltransferase 1B; histone H3-K9 methyltransferase 2
UniProt Protein Name:	Histone-lysine N-methyltransferase SUV39H2
UniProt Synonym Protein Names:	Histone H3-K9 methyltransferase 2; H3-K9-HMTase 2; Lysine N-methyltransferase 1B; Suppressor of variegation 3-9 homolog 2; Su(var)3-9 homolog 2
Protein Family:	Histone-lysine N-methyltransferase
UniProt Gene Name:	SUV39H2
UniProt Entry Name:	SUV92_HUMAN

	Western blot analysis of extracts of various cell lines, using SUV39H2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
	Chromatin immunoprecipitation analysis of extracts of 293T cell line, using SUV39H2 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.