Antibody Name:	Anti-SUV39H1 Antibody
Antibody SKU:	CAB3277
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP ChIP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SUV39H1 (NP_003164.1).

Application:	WB IHC IF IP ChIP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 ChIP 1:20 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SUV39H1 (NP_003164.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAEN LKGC SVCC KSSW NQLQ DLCR LAKL SCPA LGIS KRNL YDFE VEYL CDYK KIRE QEYY LVKW RGYP DSES TWEP RQNL KCVR ILKQ FHKD LERE LLRR HHRS KTPR HLDP SLAN YLVQ KAKQ RRAL RRWE QELN AKRS HLGR ITVE NEVD LDGP PRAF VYIN EYRV GEGI TLNQ VAVG CECQ DCLW APTG GCCP GASL HKFA YNDQ GQVR LRAG LPIY
Gene ID:	6839
Uniprot:	O43463
Cellular Location:	Chromosome, Nucleus, Nucleus lamina, centromere, nucleoplasm
Calculated MW:	47kDa/49kDa
Observed MW:	48KDa

Synonyms:

SUV39H1, H3-K9-HMTase 1, KMT1A, MG44, SUV39H, H3-K9-HMTase1

Background:

This gene encodes an evolutionarily-conserved protein containing an N-terminal chromodomain and a C-terminal SET domain. The encoded protein is a histone methyltransferase that trimethylates lysine 9 of histone H3, which results in transcriptional gene silencing. Loss of function of this gene disrupts heterochromatin formation and may cause chromosome instability. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	SUV39H1: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys- 9' as substrate. Also weakly methylates histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone- modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Interacts with H3 and H4 histones. Interacts with GFI1B, DNMT3B, CBX1, CBX4, KIAA1967/DBC1, MBD1, RUNX1, RUNX3, MYOD1, SMAD5 and RB1. Interacts with SBF1 through the SET domain. Interacts with HDAC1 and HDAC2 through the N-terminus and associates with the core histone deacetylase complex composed of HDAC1, HDAC2, RBBP4 and RBBP7. Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8. In case of infection, interacts with HTLV-1 Tax protein, leading to abrogate Tax transactivation of HTLV-1 LTR. Interacts (via SET domain) with MECOM; enhances MECOM transcriptional repression activity. Inhibited by S-adenosyl-L-homocysteine. Negatively regulated by KIAA1967/DBC1. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily.
UniProt Protein Details:	Protein type:Methyltransferase, protein lysine; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation; Methyltransferase Chromosomal Location of Human Ortholog: Xp11.23 Cellular Component: chromatin silencing complex; condensed nuclear chromosome; heterochromatin; nucleoplasm; nucleus Molecular Function:chromatin binding; histone lysine N-methyltransferase activity (H3-K9 specific); histone methyltransferase activity; histone-lysine N-methyltransferase activity; protein binding; protein N-terminus binding; S-adenosylmethionine-dependent methyltransferase activity Biological Process: chromatin silencing at rDNA; establishment and/or maintenance of chromatin architecture; negative regulation of circadian rhythm; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; response to DNA damage stimulus
NCBI Summary:	This gene encodes an evolutionarily-conserved protein containing an N-terminal chromodomain and a C-terminal SET domain. The encoded protein is a histone methyltransferase that trimethylates lysine 9 of histone H3, which results in transcriptional gene silencing. Loss of function of this gene disrupts heterochromatin formation and may cause chromosome instability. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
UniProt Code:	O43463
NCBI GenInfo Identifier:	25091290
NCBI Gene ID:	6839
NCBI Accession:	O43463.1
UniProt Secondary Accession:	O43463,Q53G60, Q6FHK6, B2R6E8, B4DST0,
UniProt Related Accession:	O43463
Molecular Weight:	49,148 Da
NCBI Full Name:	Histone-lysine N-methyltransferase SUV39H1
NCBI Synonym Full Names:	suppressor of variegation 3-9 homolog 1
NCBI Official Symbol:	SUV39H1Â Â
NCBI Official Synonym Symbols:	MG44; KMT1A; SUV39H; H3-K9-HMTase 1Â Â
NCBI Protein Information:	histone-lysine N-methyltransferase SUV39H1
UniProt Protein Name:	Histone-lysine N-methyltransferase SUV39H1
UniProt Synonym Protein Names:	Histone H3-K9 methyltransferase 1; H3-K9-HMTase 1; Lysine N-methyltransferase 1A; Position-effect variegation 3-9 homolog; Suppressor of variegation 3-9 homolog 1; Su(var)3-9 homolog 1
Protein Family:	Histone-lysine N-methyltransferase
UniProt Gene Name:	SUV39H1Â Â
UniProt Entry Name:	SUV91_HUMAN

Western blot analysis of extracts of various cell lines, using SUV39H1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Antibodies

SUV39H1 Antibody (PACO12590)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)