Cell Biology Antibodies 7
Anti-SURF4 Antibody (CAB16744)
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- SKU:
- CAB16744
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SURF4 Antibody |
| Antibody SKU: | CAB16744 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SURF4 (NP_001267719.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, mouse brain, rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SURF4 (NP_001267719.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ACFG LFGI IALQ TIAY SILW DLKF LMRN LALG GGLL LLLA ESRS EGKS MFAG VPTM RESS PKQY MQLG GRVL LVLM FMTL LHFD ASFF SIVQ NIVG TALM I |
| Gene ID: | 6836 |
| Uniprot: | O15260 |
| Cellular Location: | |
| Calculated MW: | 30kDa |
| Observed MW: | 30kDa |
| Synonyms: | SURF4, ERV29, surfeit 4 |
| Background: | This gene is located in the surfeit gene cluster, which is comprised of very tightly linked housekeeping genes that do not share sequence similarity. The encoded protein is a conserved integral membrane protein that interacts with endoplasmic reticulum-Golgi intermediate compartment proteins. Disruption of this gene results in reduced numbers of endoplasmic reticulum-Golgi intermediate compartment clusters and redistribution of coat protein I to the cytosol. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | SURF4: May play a role in the maintenance of the architecture of the endoplasmic reticulum-Golgi intermediate compartment and of the Golgi. Belongs to the SURF4 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: 9q34.2 Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment; Golgi membrane; transport vesicle Molecular Function:protein binding Biological Process: Golgi organization and biogenesis; protein exit from endoplasmic reticulum; retrograde vesicle-mediated transport, Golgi to ER |
| NCBI Summary: | This gene is located in the surfeit gene cluster, which is comprised of very tightly linked housekeeping genes that do not share sequence similarity. The encoded protein is a conserved integral membrane protein that interacts with endoplasmic reticulum-Golgi intermediate compartment proteins. Disruption of this gene results in reduced numbers of endoplasmic reticulum-Golgi intermediate compartment clusters and redistribution of coat protein I to the cytosol. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| UniProt Code: | O15260 |
| NCBI GenInfo Identifier: | 12644052 |
| NCBI Gene ID: | 6836 |
| NCBI Accession: | O15260.3 |
| UniProt Secondary Accession: | O15260,O60923, Q5T8U6, Q9UNZ0, Q9UNZ1, B7Z6A4, |
| UniProt Related Accession: | O15260 |
| Molecular Weight: | 14,854 Da |
| NCBI Full Name: | Surfeit locus protein 4 |
| NCBI Synonym Full Names: | surfeit 4 |
| NCBI Official Symbol: | SURF4 |
| NCBI Official Synonym Symbols: | ERV29 |
| NCBI Protein Information: | surfeit locus protein 4 |
| UniProt Protein Name: | Surfeit locus protein 4 |
| Protein Family: | Surfeit locus protein |
| UniProt Gene Name: | SURF4 |
| UniProt Entry Name: | SURF4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SURF4 antibody (CAB16744) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human liver cancer using SURF4 antibody (CAB16744) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat pancreatic islet using SURF4 antibody (CAB16744) at dilution of 1:200 (40x lens). |
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