Antibody Name:	Anti-SURF1 Antibody
Antibody SKU:	CAB12521
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-275 of human SURF1 (NP_003163.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, HL-60, BT-474, SKOV3, Mouse liver, Mouse kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-275 of human SURF1 (NP_003163.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QVQR RKWK LNLI AELE SRVL AEPV PLPA DPME LKNL EYRP VKVR GCFD HSKE LYMM PRTM VDPV REAR EGGL ISSS TQSG AYVV TPFH CTDL GVTI LVNR GFVP RKKV NPET RQKG QIEG EVDL IGMV RLTE TRQP FVPE NNPE RNHW HYRD LEAM ARIT GAEP IFID ANFQ STVP GGPI GGQT RVTL RNEH LQYI
Gene ID:	6834
Uniprot:	Q15526
Cellular Location:	Mitochondrion inner membrane, Multi-pass membrane protein
Calculated MW:	31kDa/33kDa
Observed MW:	33kDa

Synonyms:

SURF1, CMT4K

Background:

This gene encodes a protein localized to the inner mitochondrial membrane and thought to be involved in the biogenesis of the cytochrome c oxidase complex. The protein is a member of the SURF1 family, which includes the related yeast protein SHY1 and rickettsial protein RP733. The gene is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity, where it shares a bidirectional promoter with SURF2 on the opposite strand. Defects in this gene are a cause of Leigh syndrome, a severe neurological disorder that is commonly associated with systemic cytochrome c oxidase deficiency.

UniProt Protein Function:	SURF1: Probably involved in the biogenesis of the COX complex. Defects in SURF1 are a cause of Leigh syndrome (LS). LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions that is commonly associated with systemic cytochrome c oxidase (COX) deficiency. Belongs to the SURF1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Oxidoreductase; Membrane protein, multi-pass; Membrane protein, integral; Mitochondrial Chromosomal Location of Human Ortholog: 9q34.2 Cellular Component: integral to membrane; mitochondrial respiratory chain Molecular Function:protein binding; cytochrome-c oxidase activity Biological Process: ATP biosynthetic process; respiratory chain complex IV assembly; aerobic respiration; oxidative phosphorylation Disease: Leigh Syndrome
NCBI Summary:	This gene encodes a protein localized to the inner mitochondrial membrane and thought to be involved in the biogenesis of the cytochrome c oxidase complex. The protein is a member of the SURF1 family, which includes the related yeast protein SHY1 and rickettsial protein RP733. The gene is located in the surfeit gene cluster, a group of very tightly linked genes that do not share sequence similarity, where it shares a bidirectional promoter with SURF2 on the opposite strand. Defects in this gene are a cause of Leigh syndrome, a severe neurological disorder that is commonly associated with systemic cytochrome c oxidase deficiency. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15526
NCBI GenInfo Identifier:	2498973
NCBI Gene ID:	6834
NCBI Accession:	Q15526.1
UniProt Secondary Accession:	Q15526,Q5T8T3, Q5T8T4,
UniProt Related Accession:	Q15526
Molecular Weight:	300
NCBI Full Name:	Surfeit locus protein 1
NCBI Synonym Full Names:	surfeit 1
NCBI Official Symbol:	SURF1
NCBI Protein Information:	surfeit locus protein 1
UniProt Protein Name:	Surfeit locus protein 1
Protein Family:	SURF1-like protein
UniProt Gene Name:	SURF1
UniProt Entry Name:	SURF1_HUMAN

	Western blot analysis of extracts of various cell lines, using SURF1 antibody (CAB12521) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded rat kidney using SURF1 antibody (CAB12521) at dilution of 1:100 (40x lens).