Epigenetics & Nuclear Signaling Antibodies 4

Anti-SUPT4H1 Antibody (CAB7933)

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SKU:
CAB7933
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-SUPT4H1 Antibody
Antibody SKU:CAB7933
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human SUPT4H1 (NP_003159.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:MCF7, B cells
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human SUPT4H1 (NP_003159.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MALE TVPK DLRH LRAC LLCS LVKT IDQF EYDG CDNC DAYL QMKG NREM VYDC TSSS FDGI IAMM SPED SWVS KWQR VSNF KPGV YAVS VTGR LPQG IVRE LKSR GVAY KSRD TAIK T
Gene ID:6827
Uniprot:P63272
Cellular Location:Nucleus
Calculated MW:13kDa
Observed MW:14kDa
Synonyms:SUPT4H1, SPT4, SPT4H, SUPT4H, Supt4a
Background:This gene encodes the small subunit of DRB (5, 6-dichloro-1-beta-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF) complex, which regulates mRNA processing and transcription elongation by RNA polymerase II. The encoded protein is localized to the nucleus and interacts with the large subunit (SUPT5H) to form the DSIF complex. Related pseudogenes have been identified on chromosomes 2 and 12. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
NCBI Summary:This gene encodes the small subunit of DRB (5,6-dichloro-1-beta-d-ribofuranosylbenzimidazole) sensitivity-inducing factor (DSIF) complex, which regulates mRNA processing and transcription elongation by RNA polymerase II. The encoded protein is localized to the nucleus and interacts with the large subunit (SUPT5H) to form the DSIF complex. Related pseudogenes have been identified on chromosomes 2 and 12. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]
UniProt Code:P63272
NCBI GenInfo Identifier:4507311
NCBI Gene ID:6827
NCBI Accession:NP_003159.1
UniProt Secondary Accession:P63272,Q16550, Q62387, Q6ZP89, B2R4X8, D3DTZ4,
UniProt Related Accession:P63272
Molecular Weight:12
NCBI Full Name:transcription elongation factor SPT4
NCBI Synonym Full Names:SPT4 homolog, DSIF elongation factor subunit
NCBI Official Symbol:SUPT4H1  
NCBI Official Synonym Symbols:SPT4; SPT4H; SUPT4H; Supt4a  
NCBI Protein Information:transcription elongation factor SPT4
UniProt Protein Name:Transcription elongation factor SPT4
UniProt Synonym Protein Names:DRB sensitivity-inducing factor 14 kDa subunit; DSIF p14; DRB sensitivity-inducing factor small subunit; DSIF small subunit
UniProt Gene Name:SUPT4H1  
Anti-SUPT4H1 Antibody (CAB7933)Western blot analysis of extracts of various cell lines, using SUPT4H1 Antibody (CAB7933) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
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