Autophagy Antibodies
Anti-SUPT20H Antibody (CAB7374)
- SKU:
- CAB7374
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Description
| Antibody Name: | Anti-SUPT20H Antibody |
| Antibody SKU: | CAB7374 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 580-779 of human SUPT20H (NP_001014308.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480, HL-60, Mouse testis, Mouse brain, Mouse lung, Rat kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 580-779 of human SUPT20H (NP_001014308.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | NLSG LLPS GGLL PNAL PSAM QAAS QAGV PFGL KNTS SLRP LNLL QLPG GSLI FNTL QQQQ QQLS QFTP QQPQ QPTT CSPQ QPGE QGSE QGST SQEQ ALSA QQAA VINL TGVG SFMQ SQAA VLSQ LGSA ENRP EQSL PQQR FQLS SAFQ QQQQ QIQQ LRFL QHQM AMAA AAAQ TAQL HHHR HTGS QSKS KMKR GTPT TPKF |
| Gene ID: | 55578 |
| Uniprot: | Q8NEM7 |
| Cellular Location: | Nucleus |
| Calculated MW: | 80kDa/85kDa/88kDa |
| Observed MW: | 86kDa |
| Synonyms: | SUPT20H, C13, C13orf19, FAM48A, FP757, P38IP, SPT20 |
| Background: |
| UniProt Protein Function: | FAM48A: Required for MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) activation during gastrulation. Required for down- regulation of E-cadherin during gastrulation by regulating E- cadherin protein level downstream from NCK-interacting kinase (NIK) and independently of the regulation of transcription by FGF signaling and Snail. Required for starvation- induced ATG9A trafficking during autophagy. Belongs to the FAM48 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: 13q13.3 Cellular Component: nucleolus Molecular Function:protein binding; transcription cofactor activity Biological Process: establishment and/or maintenance of chromatin architecture; autophagy; gastrulation |
| UniProt Code: | Q8NEM7 |
| NCBI GenInfo Identifier: | 334302791 |
| NCBI Gene ID: | 55578 |
| NCBI Accession: | Q8NEM7.2 |
| UniProt Secondary Accession: | Q8NEM7,Q71RF3, Q9Y6A6, E7ER46, |
| UniProt Related Accession: | Q8NEM7 |
| Molecular Weight: | 779 |
| NCBI Full Name: | Transcription factor SPT20 homolog |
| NCBI Synonym Full Names: | suppressor of Ty 20 homolog (S. cerevisiae) |
| NCBI Official Symbol: | SUPT20H |
| NCBI Official Synonym Symbols: | C13; FP757; P38IP; SPT20; FAM48A; C13orf19 |
| NCBI Protein Information: | transcription factor SPT20 homolog; protein FAM48A; tumor rejection antigen; family with sequence similarity 48, member A; transcription factor (p38 interacting protein) |
| UniProt Protein Name: | Transcription factor SPT20 homolog |
| UniProt Synonym Protein Names: | p38-interacting protein; p38IP |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | SUPT20H |
| UniProt Entry Name: | SP20H_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SUPT20H antibody (CAB7374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human colon using SUPT20H antibody (CAB7374) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human kidney using SUPT20H antibody (CAB7374) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse liver using SUPT20H antibody (CAB7374) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using SUPT20H antibody (CAB7374). Blue: DAPI for nuclear staining. |
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