| UniProt Protein Function: | SPT16: Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Belongs to the peptidase M24 family. SPT16 subfamily. Component of the FACT complex, a stable heterodimer of SSRP1 and SUPT16H. Also component of a CK2-SPT16-SSRP1 complex which forms following UV irradiation, composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Interacts with NEK9. Binds to histone H2A-H2B. Interacts with GTF2E2.Protein type: Transcription initiation complexChromosomal Location of Human Ortholog: 14q11.2Cellular Component: chromosome; nucleoplasm; nucleusMolecular Function: protein bindingBiological Process: DNA repair; DNA replication; gene expression; nucleosome disassembly; positive regulation of RNA elongation; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter; viral reproduction |
| UniProt Protein Details: | |
| NCBI Summary: | Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009] |
| UniProt Code: | Q9Y5B9 |
| NCBI GenInfo Identifier: | 74753511 |
| NCBI Gene ID: | 11198 |
| NCBI Accession: | Q9Y5B9.1 |
| UniProt Secondary Accession: | Q9Y5B9,Q6GMT8, Q6P2F1, Q6PJM1, Q9NRX0 |
| UniProt Related Accession: | Q9Y5B9 |
| Molecular Weight: | 119,914 Da |
| NCBI Full Name: | FACT complex subunit SPT16 |
| NCBI Synonym Full Names: | SPT16 homolog, facilitates chromatin remodeling subunit |
| NCBI Official Symbol: | SUPT16H |
| NCBI Official Synonym Symbols: | CDC68; SPT16; FACTP140; SPT16/CDC68 |
| NCBI Protein Information: | FACT complex subunit SPT16 |
| UniProt Protein Name: | FACT complex subunit SPT16 |
| UniProt Synonym Protein Names: | Chromatin-specific transcription elongation factor 140 kDa subunit; FACT 140 kDa subunit; FACTp140; Facilitates chromatin transcription complex subunit SPT16; hSPT16 |
| Protein Family: | |
| UniProt Gene Name: | SUPT16H |
| UniProt Entry Name: | SP16H_HUMAN |
