Antibody Name:	Anti-SUN2 Antibody
Antibody SKU:	CAB16325
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human SUN2

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, U-251MG, Mouse heart

Immunogen:	Recombinant protein of human SUN2
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	25777
Uniprot:	Q9UH99
Cellular Location:
Calculated MW:	79kDa/80kDa/82kDa
Observed MW:	80kDa

Synonyms:

SUN2, UNC84B

Background:

SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope, known as the LINC complex, via interaction with the conserved luminal KASH domain of nesprins (e.g., SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton, which contributes to nuclear positioning and cellular rigidity (summary by Haque et al., 2010 [PubMed 19933576]).

UniProt Protein Function:	SUN2: a nuclear transmembrane protein. Forms part of a membrane-spanning fibrillar complex that interconnects attached meiotic telomeres with cytoplasmic structures. Together with SUN1 connects the nuclear envelope with the centrosome. Interacts with RAB5A. Reportedly expressed in the endosome where it may play a role in endosome fusion and endocytosis.
UniProt Protein Details:	Protein type:Membrane protein, integral; Nuclear envelope Chromosomal Location of Human Ortholog: 22q13.1 Cellular Component: nuclear envelope; nuclear membrane Molecular Function:identical protein binding; lamin binding; microtubule binding; protein binding Biological Process: centrosome localization; mitotic spindle organization and biogenesis; nuclear membrane organization and biogenesis; nuclear migration; nuclear migration along microfilament; positive regulation of cell migration
NCBI Summary:	SUN1 (MIM 607723) and SUN2 are inner nuclear membrane (INM) proteins that play a major role in nuclear-cytoplasmic connection by formation of a 'bridge' across the nuclear envelope, known as the LINC complex, via interaction with the conserved luminal KASH domain of nesprins (e.g., SYNE1; MIM 608441) located in the outer nuclear membrane (ONM). The LINC complex provides a direct connection between the nuclear lamina and the cytoskeleton, which contributes to nuclear positioning and cellular rigidity (summary by Haque et al., 2010 [PubMed 19933576]).[supplied by OMIM, Nov 2010]
UniProt Code:	Q9UH99
NCBI GenInfo Identifier:	29337242
NCBI Gene ID:	25777
NCBI Accession:	Q9UH99.3
UniProt Secondary Accession:	Q9UH99,O75156, Q2NKN8, Q2T9F7, Q504T5, Q6B4H1, Q7Z3E3 B0QY62,
UniProt Related Accession:	Q9UH99
Molecular Weight:	79,612 Da
NCBI Full Name:	SUN domain-containing protein 2
NCBI Synonym Full Names:	Sad1 and UNC84 domain containing 2
NCBI Official Symbol:	SUN2
NCBI Official Synonym Symbols:	UNC84B
NCBI Protein Information:	SUN domain-containing protein 2
UniProt Protein Name:	SUN domain-containing protein 2
UniProt Synonym Protein Names:	Protein unc-84 homolog B; Rab5-interacting protein; Rab5IP; Sad1/unc-84 protein-like 2
Protein Family:	SUN domain-containing protein
UniProt Gene Name:	SUN2
UniProt Entry Name:	SUN2_HUMAN

	Western blot analysis of extracts of various cell lines, using SUN2 antibody (CAB16325) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded Human breast using SUN2 Rabbit pAb (CAB16325) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using SUN2 antibody (CAB16325) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using SUN2 antibody (CAB16325) at dilution of 1:200. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using SUN2 antibody (CAB16325) at dilution of 1:100. Blue: DAPI for nuclear staining.