Antibody Name:	Anti-SULT1E1 Antibody
Antibody SKU:	CAB7452
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 140-294 of human SULT1E1 (NP_005411.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 140-294 of human SULT1E1 (NP_005411.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YFFL MVAG HPNP GSFP EFVE KFMQ GQVP YGSW YKHV KSWW EKGK SPRV LFLF YEDL KEDI RKEV IKLI HFLE RKPS EELV DRII HHTS FQEM KNNP STNY TTLP DEIM NQKL SPFM RKGI TGDW KNHF TVAL NEKF DKHY EQQM KEST LKFR TEI
Gene ID:	6783
Uniprot:	P49888
Cellular Location:	Cytoplasm
Calculated MW:	35kDa
Observed MW:	35kDa

Synonyms:

SULT1E1, EST, EST-1, ST1E1, STE

Background:

Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors.

UniProt Protein Function:	SULT1E1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 20 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated. Belongs to the sulfotransferase 1 family.
UniProt Protein Details:	Protein type:EC 2.8.2.4; Transferase; Energy Metabolism - sulfur; Lipid Metabolism - androgen and estrogen Chromosomal Location of Human Ortholog: 4q13.1 Cellular Component: cytosol Molecular Function:steroid sulfotransferase activity; estrone sulfotransferase activity; flavonol 3-sulfotransferase activity; steroid binding Biological Process: steroid metabolic process; estrogen metabolic process; xenobiotic metabolic process; sulfation; female pregnancy; 3'-phosphoadenosine 5'-phosphosulfate metabolic process
NCBI Summary:	Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a protein that transfers a sulfo moiety to and from estrone, which may control levels of estrogen receptors. [provided by RefSeq, Jul 2008]
UniProt Code:	P49888
NCBI GenInfo Identifier:	1711604
NCBI Gene ID:	6783
NCBI Accession:	P49888.1
UniProt Related Accession:	P49888
Molecular Weight:	35kDa
NCBI Full Name:	Estrogen sulfotransferase
NCBI Synonym Full Names:	sulfotransferase family 1E member 1
NCBI Official Symbol:	SULT1E1
NCBI Official Synonym Symbols:	EST; STE; EST-1; ST1E1
NCBI Protein Information:	estrogen sulfotransferase
UniProt Protein Name:	Estrogen sulfotransferase
UniProt Synonym Protein Names:	EST-1; Sulfotransferase 1E1; ST1E1; Sulfotransferase, estrogen-preferring
Protein Family:	Estrogen sulfotransferase
UniProt Gene Name:	SULT1E1
UniProt Entry Name:	ST1E1_HUMAN

	Western blot analysis of extracts of various cell lines, using SULT1E1 antibody (CAB7452) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using SULT1E1 antibody (CAB7452). Blue: DAPI for nuclear staining.