Name: Anti-SUFU Antibody (CAB13429)
Brand: Cell Biology Antibodies 4
SKU: CAB13429
Availability: OutOfStock

Antibody Name:	Anti-SUFU Antibody
Antibody SKU:	CAB13429
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 325-484 of human SUFU (NP_057253.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 325-484 of human SUFU (NP_057253.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PPIN PQRQ NGLA HDRA PSRK DSLE SDSS TAII PHEL IRTR QLES VHLK FNQE SGAL IPLC LRGR LLHG RHFT YKSI TGDM AITF VSTG VEGA FATE EHPY AAHG PWLQ ILLT EEFV EKML EDLE DLTS PEEF KLPK EYSW PEKK LKVS ILPD VVFD SPLH
Gene ID:	51684
Uniprot:	Q9UMX1
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	47kDa/52kDa/53kDa
Observed MW:	54kDa

Synonyms:	SUFU, PRO1280, SUFUH, SUFUXL
Background:	The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	SUFU: a transcriptional regulator which negatively regulates the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. Modulates nuclear-cytoplasmic shuttling of Gli-1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of FUSED to both SUFU and a GLI protein. Mutations in SUFU predispose to medulloblastoma. Part of a DNA-bound corepressor complex containing SAP18, GLI1 and SIN3. Part of a complex containing CTNNB1. Binds BTRC, GLI2, GLI3, SAP18 and STK36. Binds both free and DNA-bound GLI1. Defects in SUFU are a cause of medulloblastoma (MDB). MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children. Three alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor; Adaptor/scaffold; Tumor suppressor Chromosomal Location of Human Ortholog: 10q24.32 Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol Molecular Function:signal transducer activity; protein binding; beta-catenin binding; transcription factor binding; transcription corepressor activity; protein kinase binding Biological Process: skin development; cytoplasmic sequestering of transcription factor; multicellular organismal development; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription factor import into nucleus; proteolysis; signal transduction; regulation of transcription, DNA-dependent; negative regulation of smoothened signaling pathway; neural tube closure; negative regulation of osteoblast differentiation; heart looping; skeletal development; smoothened signaling pathway involved in ventral spinal cord interneuron specification; smoothened signaling pathway involved in spinal cord motor neuron cell fate specification Disease: Meningioma, Familial, Susceptibility To; Basal Cell Nevus Syndrome; Medulloblastoma
NCBI Summary:	The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
UniProt Code:	Q9UMX1
NCBI GenInfo Identifier:	62511179
NCBI Gene ID:	51684
NCBI Accession:	Q9UMX1.2
UniProt Secondary Accession:	Q9UMX1,Q7LCP7, Q9NT90, Q9NZ07, Q9UHK2, Q9UHM8, Q9UMY0
UniProt Related Accession:	Q9UMX1
Molecular Weight:	484
NCBI Full Name:	Suppressor of fused homolog
NCBI Synonym Full Names:	suppressor of fused homolog (Drosophila)
NCBI Official Symbol:	SUFU
NCBI Official Synonym Symbols:	SUFUH; SUFUXL; PRO1280
NCBI Protein Information:	suppressor of fused homolog
UniProt Protein Name:	Suppressor of fused homolog
Protein Family:	Suppressor of fused
UniProt Gene Name:	SUFU
UniProt Entry Name:	SUFU_HUMAN

	Western blot analysis of extracts of various cell lines, using SUFU antibody (CAB13429) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human breast cancer using SUFU Rabbit pAb (CAB13429) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal cancer using SUFU Rabbit pAb (CAB13429) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of MCF-7 cells using SUFU antibody (CAB13429). Blue: DAPI for nuclear staining.