Name: Anti-SUCLG1 Antibody (CAB15345)
Brand: Metabolism Antibodies 1
SKU: CAB15345
Availability: OutOfStock

Antibody Name:	Anti-SUCLG1 Antibody
Antibody SKU:	CAB15345
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 41-346 of human SUCLG1 (NP_003840.2).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, HepG2, A-549, Mouse thymus, Mouse brain, Mouse heart, Mouse kidney, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 41-346 of human SUCLG1 (NP_003840.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CSYT ASRQ HLYV DKNT KIIC QGFT GKQG TFHS QQAL EYGT KLVG GTTP GKGG QTHL GLPV FNTV KEAK EQTG ATAS VIYV PPPF AAAA INEA IEAE IPLV VCIT EGIP QQDM VRVK HKLL RQEK TRLI GPNC PGVI NPGE CKIG IMPG HIHK KGRI GIVS RSGT LTYE AVHQ TTQV GLGQ SLCV GIGG DPFN GTDF IDCL EIFL NDSA TEGI ILIG EIGG NAEE NAAE FLKQ HNSG PNSK PVVS FIAG LTAP PGRR MGHA GAII AGGK GGAK EKIS ALQS AGVV VSMS PAQL GTTI YKEF EKRK ML
Gene ID:	8802
Uniprot:	P53597
Cellular Location:	Mitochondrion
Calculated MW:	36kDa
Observed MW:	26-36kDa

Synonyms:	SUCLG1, GALPHA, MTDPS9, SUCLA1
Background:	This gene encodes the alpha subunit of the heterodimeric enzyme succinate coenzyme A ligase. This enzyme is targeted to the mitochondria and catalyzes the conversion of succinyl CoA and ADP or GDP to succinate and ATP or GTP. Mutations in this gene are the cause of the metabolic disorder fatal infantile lactic acidosis and mitochondrial DNA depletion.

UniProt Protein Function:	SUCLG1: Catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. The nature of the beta subunit determines the nucleotide specificity. Heterodimer of an alpha and a beta subunit. Belongs to the succinate/malate CoA ligase alpha subunit family.
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - propanoate; Mitochondrial; EC 6.2.1.4; Carbohydrate Metabolism - citrate (TCA) cycle; EC 6.2.1.5; Ligase Chromosomal Location of Human Ortholog: 2p11.2 Cellular Component: mitochondrion; mitochondrial matrix; mitochondrial inner membrane; cytoplasm; plasma membrane Molecular Function:succinate-CoA ligase (GDP-forming) activity; GDP binding; GTP binding; ATP citrate synthase activity; protein heterodimerization activity; cofactor binding; succinate-CoA ligase (ADP-forming) activity Biological Process: cellular metabolic process; succinate metabolic process; tricarboxylic acid cycle; succinyl-CoA metabolic process Disease: Mitochondrial Dna Depletion Syndrome 9 (encephalomyopathic Type With Methylmalonic Aciduria)
NCBI Summary:	This gene encodes the alpha subunit of the heterodimeric enzyme succinate coenzyme A ligase. This enzyme is targeted to the mitochondria and catalyzes the conversion of succinyl CoA and ADP or GDP to succinate and ATP or GTP. Mutations in this gene are the cause of the metabolic disorder fatal infantile lactic acidosis and mitochondrial DNA depletion. [provided by RefSeq, Feb 2010]
UniProt Code:	P53597
NCBI GenInfo Identifier:	1122286
NCBI Gene ID:	8802
NCBI Accession:	CAA92426.1
UniProt Secondary Accession:	P53597,Q9BWB0, Q9UNP6,
UniProt Related Accession:	P53597
Molecular Weight:	36,250 Da
NCBI Full Name:	succinyl coenzyme A synthetase
NCBI Synonym Full Names:	succinate-CoA ligase, alpha subunit
NCBI Official Symbol:	SUCLG1
NCBI Official Synonym Symbols:	GALPHA; MTDPS9; SUCLA1
NCBI Protein Information:	succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial; succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial; SCS-alpha; succinyl-CoA synthetase subunit alpha; succinyl-CoA ligase [GDP-forming] subunit alpha, mitochondrial
UniProt Protein Name:	Succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial
UniProt Synonym Protein Names:	Succinyl-CoA synthetase subunit alpha
UniProt Gene Name:	SUCLG1
UniProt Entry Name:	SUCA_HUMAN

	Western blot analysis of extracts of various cell lines, using SUCLG1 antibody (CAB15345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of C6 cells using SUCLG1 antibody (CAB15345) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using SUCLG1 antibody (CAB15345) at dilution of 1:100. Blue: DAPI for nuclear staining.