Metabolism Antibodies 1

Anti-SUCLA2 Antibody (CAB10040)

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SKU:
CAB10040
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-SUCLA2 Antibody
Antibody SKU:CAB10040
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human SUCLA2 (NP_003841.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human, Mouse, Rat
Positive Samples:293T, SW480, HeLa, Mouse heart, Mouse liver, Mouse brain, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human SUCLA2 (NP_003841.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAS MFYG RLVA VATL RNHR PRTA QRAA AQVL GSSG LFNN HGLQ VQQQ QQRN LSLH EYMS MELL QEAG VSVP KGYV AKSP DEAY AIAK KLGS KDVV IKAQ VLAG GRGK GTFE SGLK GGVK IVFS PEEA KAVS SQMI GKKL FTKQ TGEK GRIC NQVL VCER KYPR REYY FAIT MERS FQGP
Gene ID:8803
Uniprot:Q9P2R7
Cellular Location:Mitochondrion
Calculated MW:48kDa/50kDa
Observed MW:50kDa
Synonyms:SUCLA2, A-BETA, A-SCS, MTDPS5, SCS-betaA
Background:Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6.
UniProt Protein Function:SCS-beta: a mitochondrial enzyme that catalyzes the phosphorylation of NTP, where N = adenosine or guanosine, in the tricarboxylic acid cycle. Catalyzes the following: ATP + succinate + CoA = ADP + succinyl-CoA + phosphate. Two splice variant isoforms have been described.
UniProt Protein Details:

Protein type:Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; EC 6.2.1.5; Carbohydrate Metabolism - propanoate; Ligase

Chromosomal Location of Human Ortholog: 13q14.2

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function:protein binding; metal ion binding; succinate-CoA ligase (ADP-forming) activity; ATP binding

Biological Process: succinate metabolic process; cellular metabolic process; succinyl-CoA pathway; succinyl-CoA metabolic process; tricarboxylic acid cycle

Disease: Mitochondrial Dna Depletion Syndrome 5 (encephalomyopathic With Or Without Methylmalonic Aciduria)

NCBI Summary:Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6. [provided by RefSeq, Jul 2008]
UniProt Code:Q9P2R7
NCBI GenInfo Identifier:94730427
NCBI Gene ID:8803
NCBI Accession:Q9P2R7.3
UniProt Secondary Accession:Q9P2R7,O95194, Q5T9Q4, Q5T9Q6, Q9NV21, Q9NVP7, B2RDE7
UniProt Related Accession:Q9P2R7
Molecular Weight:48,040 Da
NCBI Full Name:Succinyl-CoA ligase
NCBI Synonym Full Names:succinate-CoA ligase, ADP-forming, beta subunit
NCBI Official Symbol:SUCLA2  
NCBI Official Synonym Symbols:A-BETA; MTDPS5; SCS-betaA  
NCBI Protein Information:succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial
UniProt Protein Name:Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial
UniProt Synonym Protein Names:ATP-specific succinyl-CoA synthetase subunit beta; Renal carcinoma antigen NY-REN-39; Succinyl-CoA synthetase beta-A chain; SCS-betaA
UniProt Gene Name:SUCLA2  
UniProt Entry Name:SUCB1_HUMAN
Anti-SUCLA2 Antibody (CAB10040)Western blot analysis of extracts of various cell lines, using SUCLA2 antibody (CAB10040) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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