Cell Biology Antibodies 9

Anti-STXBP1 Antibody (CAB5420)

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SKU:
CAB5420
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Reactivity:
Fish - Zebrafish
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-STXBP1 Antibody
Antibody SKU:CAB5420
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat, Zebrafish
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 295-594 of human STXBP1 (NP_001027392.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat, Zebrafish
Positive Samples:HeLa, K-562, Mouse lung, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 295-594 of human STXBP1 (NP_001027392.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HIAE VSQE VTRS LKDF SSSK RMNT GEKT TMRD LSQM LKKM PQYQ KELS KYST HLHL AEDC MKHY QGTV DKLC RVEQ DLAM GTDA EGEK IKDP MRAI VPIL LDAN VSTY DKIR IILL YIFL KNGI TEEN LNKL IQHA QIPP EDSE IITN MAHL GVPI VTDS TLRR RSKP ERKE RISE QTYQ LSRW TPII KDIM EDTI EDKL DTKH YPYI STRS SASF STTA VSAR YGHW HKNK APGE YRSG PRLI IFIL GGVS LNEM RCAY EVTQ ANGK WEVL IGST HILT PQKL LDTL KKLN KTDE EISS
Gene ID:6812
Uniprot:P61764
Cellular Location:Cytoplasm, Membrane, Peripheral membrane protein, cytosol
Calculated MW:67kDa/68kDa
Observed MW:68kDa
Synonyms:STXBP1, MUNC18-1, NSEC1, P67, RBSEC1, UNC18
Background:This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described.
UniProt Protein Function:STXBP1: may participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. Two splice-variant isoforms have been described.
UniProt Protein Details:

Protein type:Vesicle

Chromosomal Location of Human Ortholog: 9q34.1

Cellular Component: cytoplasm; cytosol; mitochondrion; myelin sheath; nucleoplasm; plasma membrane; protein complex; terminal button

Molecular Function:identical protein binding; protein binding; protein domain specific binding; protein kinase binding; protein N-terminus binding; SNARE binding; syntaxin binding; syntaxin-1 binding

Biological Process: axon target recognition; energy reserve metabolic process; glutamate secretion; negative regulation of neuron apoptosis; negative regulation of synaptic transmission, GABAergic; neuromuscular synaptic transmission; neurotransmitter secretion; platelet degranulation; positive regulation of calcium ion-dependent exocytosis; protein stabilization; protein transport; regulation of insulin secretion; regulation of synaptic vesicle fusion to presynaptic membrane; synaptic transmission; synaptic vesicle maturation; vesicle docking during exocytosis

Disease: Epileptic Encephalopathy, Early Infantile, 4

NCBI Summary:This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2010]
UniProt Code:P61764
NCBI GenInfo Identifier:50403646
NCBI Gene ID:6812
NCBI Accession:P61764.1
UniProt Secondary Accession:P61764,Q28208, Q62759, Q64320, Q96TG8, B1AM97,
UniProt Related Accession:P61764
Molecular Weight:68,736 Da
NCBI Full Name:Syntaxin-binding protein 1
NCBI Synonym Full Names:syntaxin binding protein 1
NCBI Official Symbol:STXBP1  
NCBI Official Synonym Symbols:P67; NSEC1; UNC18; RBSEC1; MUNC18-1  
NCBI Protein Information:syntaxin-binding protein 1
UniProt Protein Name:Syntaxin-binding protein 1
UniProt Synonym Protein Names:MUNC18-1; N-Sec1; Protein unc-18 homolog 1; Unc18-1; Protein unc-18 homolog A; Unc-18A; p67
Protein Family:Syntaxin-binding protein
UniProt Gene Name:STXBP1  
UniProt Entry Name:STXB1_HUMAN
Anti-STXBP1 Antibody (CAB5420)Western blot analysis of extracts of various cell lines, using STXBP1 antibody (CAB5420) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-STXBP1 Antibody (CAB5420)Immunohistochemistry of paraffin-embedded rat brain using STXBP1 antibody (CAB5420) at dilution of 1:100 (40x lens).
Anti-STXBP1 Antibody (CAB5420)Immunohistochemistry of paraffin-embedded mouse spinal cord using STXBP1 antibody (CAB5420) at dilution of 1:100 (40x lens).
Anti-STXBP1 Antibody (CAB5420)Immunofluorescence analysis of rat brain using STXBP1 antibody (CAB5420) at dilution of 1:50. Blue: DAPI for nuclear staining.
Anti-STXBP1 Antibody (CAB5420)Immunofluorescence analysis of mouse brain using STXBP1 antibody (CAB5420) at dilution of 1:50. Blue: DAPI for nuclear staining.
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system_update_altDatasheetHuman STXBP1/UNC18A Recombinant Protein Syntaxin-binding protein 1, also known as N-Sec1, Protein unc-18 homolog 1, MUNC18-1 and STXBP1, is a peripheral membrane prote