Cell Biology Antibodies 9
Anti-STX1A Antibody (CAB5570)
- SKU:
- CAB5570
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-STX1A Antibody |
| Antibody SKU: | CAB5570 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-288 of human STX1A (NP_004594.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Rat |
| Positive Samples: | U-251MG, HT-1080, BT-474 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-288 of human STX1A (NP_004594.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MKDR TQEL RTAK DSDD DDDV AVTV DRDR FMDE FFEQ VEEI RGFI DKIA ENVE EVKR KHSA ILAS PNPD EKTK EELE ELMS DIKK TANK VRSK LKSI EQSI EQEE GLNR SSAD LRIR KTQH STLS RKFV EVMS EYNA TQSD YRER CKGR IQRQ LEIT GRTT TSEE LEDM LESG NPAI FASG IIMD SSIS KQAL SEIE TRHS EIIK LENS IREL HDMF MDMA MLVE SQGE MIDR IEYN VEHA VDYV ERAV SDTK KAVK YQSK ARRK KIMI IICC VILG IVIA STVG GIFA |
| Gene ID: | 6804 |
| Uniprot: | Q16623 |
| Cellular Location: | Cell junction, Cell membrane, Cytoplasmic vesicle, Secreted, Single-pass type IV membrane protein, secretory vesicle, synapse, synaptic vesicle membrane, synaptosome |
| Calculated MW: | 28kDa/29kDa/33kDa |
| Observed MW: | 33kDa |
| Synonyms: | STX1A, HPC-1, P35-1, STX1, SYN1A, syntaxin-1A |
| Background: | This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | STX1A: a type IV membrane protein involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. Three splice variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Vesicle Cellular Component: actomyosin; cell junction; cytoplasmic vesicle; endomembrane system; integral to membrane; intracellular organelle; membrane; neuron projection; plasma membrane; protein complex; secretory granule; SNARE complex; synapse; synaptic vesicle; synaptic vesicle membrane; voltage-gated potassium channel complex Molecular Function:ATP-dependent protein binding; calcium channel inhibitor activity; calcium-dependent protein binding; chloride channel inhibitor activity; glycoprotein binding; kinase binding; myosin binding; myosin head/neck binding; protein binding; protein binding, bridging; protein domain specific binding; protein heterodimerization activity; protein N-terminus binding; SNAP receptor activity; SNARE binding Biological Process: calcium ion-dependent exocytosis; exocytosis; intracellular protein transport; neurotransmitter transport; positive regulation of calcium ion-dependent exocytosis; positive regulation of catecholamine secretion; positive regulation of exocytosis; positive regulation of neurotransmitter secretion; regulation of exocytosis; response to gravity; secretion by cell; synaptic vesicle fusion to presynaptic membrane; transport; vesicle docking; vesicle-mediated transport |
| NCBI Summary: | This gene encodes a member of the syntaxin superfamily. Syntaxins are nervous system-specific proteins implicated in the docking of synaptic vesicles with the presynaptic plasma membrane. Syntaxins possess a single C-terminal transmembrane domain, a SNARE [Soluble NSF (N-ethylmaleimide-sensitive fusion protein)-Attachment protein REceptor] domain (known as H3), and an N-terminal regulatory domain (Habc). Syntaxins bind synaptotagmin in a calcium-dependent fashion and interact with voltage dependent calcium and potassium channels via the C-terminal H3 domain. This gene product is a key molecule in ion channel regulation and synaptic exocytosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
| UniProt Code: | Q16623 |
| NCBI GenInfo Identifier: | 2501084 |
| NCBI Gene ID: | 6804 |
| NCBI Accession: | Q16623.1 |
| UniProt Secondary Accession: | Q16623,O35526, P32851, |
| UniProt Related Accession: | Q16623 |
| Molecular Weight: | 33,054 Da |
| NCBI Full Name: | Syntaxin-1A |
| NCBI Synonym Full Names: | syntaxin 1A |
| NCBI Official Symbol: | STX1A |
| NCBI Official Synonym Symbols: | STX1; HPC-1; P35-1; SYN1A |
| NCBI Protein Information: | syntaxin-1A |
| UniProt Protein Name: | Syntaxin-1A |
| UniProt Synonym Protein Names: | Neuron-specific antigen HPC-1 |
| Protein Family: | Syntaxin |
| UniProt Gene Name: | Stx1a |
| UniProt Entry Name: | STX1A_MOUSE |
 | Western blot analysis of extracts of various cell lines, using STX1A antibody (CAB5570) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded human liver using STX1A antibody (CAB5570) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using STX1A antibody (CAB5570) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using STX1A Polyclonal Antibody (CAB5570) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
Related Products
Anti-STX1A Antibody (CAB19243)
Human Syntaxin-1A / STX1A ELISA Kit
