Cell Biology Antibodies 4
Anti-STX10 Antibody (CAB13827)
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- SKU:
- CAB13827
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-STX10 Antibody |
| Antibody SKU: | CAB13827 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 76-165 of human STX10 (NP_001258540.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 76-165 of human STX10 (NP_001258540.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GKPA AQKS PSDL LDAS AVSA TSRY IEEQ QATQ QLIM DEQD QQLE MVSG SIQV LKHM SGRV GEEL DEQG IMLD AFAQ EMDH TQSR MDGV LR |
| Gene ID: | 8677 |
| Uniprot: | O60499 |
| Cellular Location: | Golgi apparatus membrane, Single-pass type IV membrane protein |
| Calculated MW: | 22kDa/28kDa |
| Observed MW: | 35kDa |
| Synonyms: | STX10, SYN10, hsyn10 |
| Background: | This gene belongs to the syntaxin family and encodes a soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE). The encoded protein is involved in docking and fusion events at the Golgi apparatus. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | STX10: SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. Belongs to the syntaxin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Vesicle Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: integral to membrane; perinuclear region of cytoplasm; SNARE complex; trans-Golgi network; trans-Golgi network membrane; vesicle Molecular Function:protein binding; SNAP receptor activity; syntaxin binding Biological Process: intracellular protein transport; regulation of protein localization; retrograde transport, endosome to Golgi; vesicle docking; vesicle fusion |
| NCBI Summary: | This gene belongs to the syntaxin family and encodes a soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE). The encoded protein is involved in docking and fusion events at the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012] |
| UniProt Code: | O60499 |
| NCBI GenInfo Identifier: | 17368024 |
| NCBI Gene ID: | 8677 |
| NCBI Accession: | O60499.1 |
| UniProt Secondary Accession: | O60499,Q6IAP4, Q96AE8, A6NC41, |
| UniProt Related Accession: | O60499 |
| Molecular Weight: | 22,445 Da |
| NCBI Full Name: | Syntaxin-10 |
| NCBI Synonym Full Names: | syntaxin 10 |
| NCBI Official Symbol: | STX10 |
| NCBI Official Synonym Symbols: | SYN10; hsyn10 |
| NCBI Protein Information: | syntaxin-10 |
| UniProt Protein Name: | Syntaxin-10 |
| Protein Family: | Syntaxin |
| UniProt Gene Name: | STX10 |
| UniProt Entry Name: | STX10_HUMAN |
 | Western blot analysis of extracts of HeLa cells, using STX10 antibody (CAB13827) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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